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The Oxymonad Genome Displays Canonical Eukaryotic Complexity in the Absence of a Mitochondrion
Charles Univ Prague, Fac Sci, Dept Parasitol, BIOCEV, Vestec, Czech Republic;Univ Warsaw, Fac Biol, Biol & Chem Res Ctr, Dept Mol Phylogenet & Evolut, Warsaw, Poland.ORCID iD: 0000-0003-3709-7873
Charles Univ Prague, Fac Sci, Dept Parasitol, BIOCEV, Vestec, Czech Republic.
Charles Univ Prague, Fac Sci, Dept Parasitol, BIOCEV, Vestec, Czech Republic.
Charles Univ Prague, Fac Sci, Dept Parasitol, BIOCEV, Vestec, Czech Republic.ORCID iD: 0000-0001-7773-9119
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2019 (English)In: Molecular biology and evolution, ISSN 0737-4038, E-ISSN 1537-1719, Vol. 36, no 10, p. 2292-2312Article in journal (Refereed) Published
Abstract [en]

The discovery that the protist Monocercomonoides exilis completely lacks mitochondria demonstrates that these organelles are not absolutely essential to eukaryotic cells. However, the degree to which the metabolism and cellular systems of this organism have adapted to the loss of mitochondria is unknown. Here, we report an extensive analysis of the M. exilis genome to address this question. Unexpectedly, we find that M. exilis genome structure and content is similar in complexity to other eukaryotes and less "reduced" than genomes of some other protists from the Metamonada group to which it belongs. Furthermore, the predicted cytoskeletal systems, the organization of endomembrane systems, and biosynthetic pathways also display canonical eukaryotic complexity. The only apparent preadaptation that permitted the loss of mitochondria was the acquisition of the SUF system for Fe-S cluster assembly and the loss of glycine cleavage system. Changes in other systems, including in amino acid metabolism and oxidative stress response, were coincident with the loss of mitochondria but are likely adaptations to the microaerophilic and endobiotic niche rather than the mitochondrial loss per se. Apart from the lack of mitochondria and peroxisomes, we show that M. exilis is a fully elaborated eukaryotic cell that is a promising model system in which eukaryotic cell biology can be investigated in the absence of mitochondria.

Place, publisher, year, edition, pages
OXFORD UNIV PRESS , 2019. Vol. 36, no 10, p. 2292-2312
Keywords [en]
amitochondrial eukaryote, cell biology, Monocercomonoides, oxymonads, protist genomics
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-400822DOI: 10.1093/molbev/msz147ISI: 000501734200017PubMedID: 31387118OAI: oai:DiVA.org:uu-400822DiVA, id: diva2:1384938
Funder
EU, Horizon 2020, 771592Available from: 2020-01-13 Created: 2020-01-13 Last updated: 2020-01-13Bibliographically approved

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Eme, LauraStairs, Courtney W.

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