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Elucidating the phylogeny of the serious plant pathogen Piptoporellus baudonii using a multigene molecular dataset
University of Dar es Salaam, Department of molecular Biology and Biotechnology.ORCID iD: 0000-0002-3105-8374
Systematisk biologi, Systematic Biology. (Tibell)ORCID iD: 0000-0003-3442-9262
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(English)Manuscript (preprint) (Other academic)
Abstract [en]

Piptoporellus baudonii is proposed as a new combination for Laetiporus baudonii in Polyporales (Basidiomycota), based on morphological and molecular features. This parasitic macrofungus attacks cashew trees, Eucalyptus, cassava, Tectona and some indigenous trees in the Southern regions of Tanzania and poses a serious threat to agroforestry and livelihood conditions in the area. Maximum likelihood and Bayesian methods were applied to obtain phylogenetic trees for partial sequences of the 5.8S part of nuc rDNA internal transcribed spacer, parts of 28S nuc rDNA (28S) and 18S nuc rDNA (18S), in addition to parts of gene encoding the translation elongation factor 1-α (TEF1) of Laetiporus baudonii for comparisons with additional sequences from the antrodia clade. Our results reveal a strongly supported phylogenetic clade of L. baudonii with Piptoporellus in Fomitopsidaceae. Piptoporellus baudonii shares many morphological features with other members of Piptoporellus but differs in having broadly ellipsoid or rarely ovoid basidiospores, while in other Piptoporellus species the spores are cylindrical to oblong-ellipsoid. Both morphological and phylogenetic evidence, however, justify the incorporation of L. baudonii in Piptoporellus, to join the three other species in the genus.

Keywords [en]
Africa, parasitic fungus, antrodia clade, Laeptiporus baudonii, macrofungus, Piptoporellus, 1 new taxon
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:uu:diva-405385OAI: oai:DiVA.org:uu-405385DiVA, id: diva2:1399538
Available from: 2020-02-28 Created: 2020-02-28 Last updated: 2020-02-28
In thesis
1. Polyporoid fungi of Tanzania: Taxonomy, transcriptomics and biochemical analyses of Kusaghiporia usambarensis and Piptoporellus baudonii
Open this publication in new window or tab >>Polyporoid fungi of Tanzania: Taxonomy, transcriptomics and biochemical analyses of Kusaghiporia usambarensis and Piptoporellus baudonii
2020 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Polyporoid fungi refers to basidiomycetes with fruiting bodies with the hymenium located to the inner surfaces of pores or narrow tubes. The majority of polyporoids belongs to Polyporales. Most Polyporales are saprobes, but some are plant pathogens. The overall aim of this thesis was to study the taxonomy, systematics and chemistry of the two species Kusaghiporia usambarensis (saprobic) and Piptoporellus baudonii (a plant pathogen) collected from Tanzania, using morphological and molecular approaches, combined with transcriptomics and pharmacognostic investigations.

The main contribution of this thesis includes the description a new genus with the new species K. usambarensis from the Usambara Mountains, Tanzania; investigation of the chemical composition of volatile compounds from this medicinal mushroom; isolation and structure determination of a novel and most abundant peptide in K. usambarensis, and further to elucidate the phylogenetic position of Piptoporellus baudonii (formerly known as Laetiporus baudonii) by using a four molecular markers dataset.

Paper I was conducted applying a classical taxonomic approach, including both morphological and phylogenetic analyses, to describe a new genus and species K. usambarensis. Paper II, investigated volatiles and volatile derivatives in dichloromethane extracts of K. usambarensis analysed by GC-MS and NMR spectroscopy. The main elements were phenols, and esters, compounds that may explain the formerly reported antioxidant activity and traditional medicinal use of the mushroom. In paper III, screening of peptides in K. usambarensis revealed a novel cysteine-rich peptide, highly expressed at gene level and the most abundant compound in the fruiting body. Combined LC-MS and transcriptome analyses were used to determine the peptide sequence, and subsequently NMR spectroscopy to determine the 3D structure of the novel peptide, kusaghitide. In paper IV molecular techniques were used to elucidate the phylogenetic position of the parasitic Laetiporus baudonii. Phylogenetic analyses of combined 5.8S, nrLSU, nrSSU, and TEF1 gene sequences placed L. baudonii in the genus Piptoporellus, hence the new combination Piptoporellus baudonii was proposed. This thesis has contributed to build capacity in the fields of mycology, systematics and pharmacognosy in order to reinforce ecological knowledge and ethnopharmaceutical research for future drug discovery in Tanzania and Africa at large.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2020. p. 56
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 1908
Keywords
Systematics, Laetiporaceae, pharmacognosy, cysteine-rich peptide, baudonii
National Category
Biological Sciences
Research subject
Biology with specialization in Systematics
Identifiers
urn:nbn:se:uu:diva-405548 (URN)978-91-513-0881-4 (ISBN)
Public defence
2020-04-17, Ekmansalen, Evolutionsbiologiskt centrum (EBC), Norbyvägen 14-18, Uppsala, 10:00 (English)
Opponent
Supervisors
Available from: 2020-03-26 Created: 2020-02-28 Last updated: 2020-03-26

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