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Carbazate modified dextrans as scavengers for carbonylated proteins
Guangxi Med Univ, Guangxi Engn Ctr Biomed Mat Tissue & Organ Regene, Affiliated Hosp 1, Nanning 530021, Peoples R China;Guangxi Med Univ, Affiliated Hosp 1, Guangxi Collaborat Innovat Ctr Biomed, Nanning 530021, Peoples R China.
Guangxi Med Univ, Guangxi Engn Ctr Biomed Mat Tissue & Organ Regene, Affiliated Hosp 1, Nanning 530021, Peoples R China;Guangxi Med Univ, Affiliated Hosp 1, Guangxi Collaborat Innovat Ctr Biomed, Nanning 530021, Peoples R China.
Guangxi Med Univ, Guangxi Engn Ctr Biomed Mat Tissue & Organ Regene, Affiliated Hosp 1, Nanning 530021, Peoples R China;Guangxi Med Univ, Affiliated Hosp 1, Guangxi Collaborat Innovat Ctr Biomed, Nanning 530021, Peoples R China;Guangxi Med Univ, Pharmaceut Coll, Nanning 530021, Peoples R China.
Guangxi Med Univ, Affiliated Hosp 1, Guangxi Collaborat Innovat Ctr Biomed, Nanning 530021, Peoples R China;Guangxi Med Univ, Pharmaceut Coll, Nanning 530021, Peoples R China.
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2020 (English)In: Carbohydrate Polymers, ISSN 0144-8617, E-ISSN 1879-1344, Vol. 232, article id 115802Article in journal (Refereed) Published
Abstract [en]

A series of biocompatible and non- toxic polysaccharide molecules have been successfully fabricated and explored their potential application for scavenging the carbonyl species in vitro. These macromolecules were dextrans with different hydrazide substitution ratios determined by TNBS assay, NMR and FTIR characterization. The colorimetric assay had demonstrated that these macromolecules could effectively scavenge acrolein, oxidized bovine serum albumin (BSA) in buffer solutions as well as carbonyl proteins from serum. The scavengers could achieve twice more scavenging effects for modified dextrans with high molecular weight (Mw=100,000) than those of low ones (Mw=40,000) with the same substitution ratio. Protein gel electrophoresis confirmed that the formation of the complex between carbonyls and modified dextrans resulted in appearance of slower bands. It also revealed that such macromolecules could protect cultured cells against the toxicity of acrolein or its derivatives. The proposed macromolecules indicated a very promising capability as scavengers for oxidative stress plus its derivatives without side effects.

Place, publisher, year, edition, pages
ELSEVIER SCI LTD , 2020. Vol. 232, article id 115802
Keywords [en]
Oxidative stress, Protein carbonyl groups, Human blood serum, Hydrazide-carbonyl click chemistry, Protein gel electrophoresis, Carbonyl scavengers
National Category
Organic Chemistry
Identifiers
URN: urn:nbn:se:uu:diva-405335DOI: 10.1016/j.carbpol.2019.115802ISI: 000507231800005PubMedID: 31952601OAI: oai:DiVA.org:uu-405335DiVA, id: diva2:1411596
Available from: 2020-03-04 Created: 2020-03-04 Last updated: 2020-03-04Bibliographically approved

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Kootala, SujitBowden, Tim

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