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Influence of nanoporesize on platelet adhesion and activation
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry. (Ytbioteknik)
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry. (Ytbioteknik.uu.se)
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Oncology, Radiology and Clinical Immunology.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry. (Ytbioteknik)
2008 (English)In: Journal of materials science. Materials in medicine, ISSN 0957-4530, E-ISSN 1573-4838, Vol. 19, no 9, p. 3115-21Article in journal (Refereed) Published
Abstract [en]

In this study we have evaluated the influence of biomaterial nano-topography on platelet adhesion and activation. Nano-porous alumina membranes with pore diameters of 20 and 200 nm were incubated with whole blood and platelet rich plasma. Platelet number, adhesion and activation were determined by using a coulter hematology analyzer, scanning electron microscopy, immunocytochemical staining in combination with light microscopy and by enzyme immunoassay. Special attention was paid to cell morphology, microparticle generation, P-selectin expression and beta-TG production. Very few platelets were found on the 200 nm alumina as compared to the 20 nm membrane. The platelets found on the 20 nm membrane showed signs of activation such as spread morphology and protruding filipodia as well as P-selectin expression. However no microparticles were detected on this surface. Despite the fact that very few platelets were found on the 200 nm alumina in contrast to the 20 nm membrane many microparticles were detected on this surface. Interestingly, all microparticles were found inside circular shaped areas of approximately 3 mum in diameter. Since this is the approximate size of a platelet we speculate that this is evidence of transient, non-adherent platelet contact with the surface, which has triggered platelet microparticle generation. To the authors knowledge, this is the first study that demonstrates how nanotexture can influence platelet microparticle generation. The study highlights the importance of understanding molecular and cellular events on nano-level when designing new biomaterials.

Place, publisher, year, edition, pages
2008. Vol. 19, no 9, p. 3115-21
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-88354DOI: 10.1007/s10856-008-3449-7ISI: 000256964300016PubMedID: 18414999OAI: oai:DiVA.org:uu-88354DiVA, id: diva2:158059
Available from: 2009-01-30 Created: 2009-01-29 Last updated: 2017-12-14Bibliographically approved
In thesis
1. Effect of Surface Nanotopography on Blood-Biomaterial Interactions
Open this publication in new window or tab >>Effect of Surface Nanotopography on Blood-Biomaterial Interactions
2010 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Biologically inspired materials are being developed with the aim of improving the integration of medical implants and minimizing non-desirable host reactions. A promising strategy is the design of topographically patterned surfaces that resemble those found in the extracellular environment.

Nanoporous alumina has been recognized as a potential biomaterial and as an important template for the fabrication of nanostructures.

In this thesis in vitro studies were done to elucidate the role of alumina nanoporosity on the inflammatory response. Specifically, by comparing alumina membranes with two pore sizes (20 and 200 nm in diameter). Complement and platelet activation were evaluated as well as monocyte/macrophage behaviour.

Whole blood was incubated with the alumina membranes and thereafter the biomaterial surfaces were evaluated in terms of protein and platelet adhesion as well as procoagulant properties. The fluid phase was analyzed for complement activation products and platelet activation markers. Besides, human mononuclear cells were cultured on the alumina membranes and cell adhesion, viability, morphology and release of pro-inflammatory cytokines were evaluated.

The results indicated that nanoporous alumina with 200 nm pores promotes higher complement activation than alumina with 20 nm pores.

In addition, platelet response to nanoporous alumina was found to be highly dependent on the material porosity, as reflected by differences in adhesion, PMP generation and procoagulant characteristics.

A clear difference in monocyte/macrophage adhesion and activation was found between the two pore size alumina membranes. Few but highly activated cells adhered to the 200 nm membrane in contrast to many but less activated monocytes/macrophages on the 20 nm surface.

The outcome of this work emphasizes that nanotopography plays an important role in the host response to biomaterials.

Better understanding of molecular interactions on nano-level will undoubtedly play a significant role in biomaterial implant development and will contribute to design strategies for controlling specific biological events.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2010. p. 74
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 693
Keywords
nanoporous alumina, nanotopography, biomaterial, platelets, complement system, macrophages, whole blood, inflammatory response
National Category
Other Basic Medicine Immunology in the medical area
Research subject
Immunology; Materials Science
Identifiers
urn:nbn:se:uu:diva-110614 (URN)978-91-554-7668-7 (ISBN)
Public defence
2010-01-20, B21, BMC, Husarg. 3, Uppsala, 09:15 (English)
Opponent
Supervisors
Available from: 2009-12-16 Created: 2009-11-18 Last updated: 2018-01-12Bibliographically approved

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