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Avian Antibodies Can Eliminate Interference Due To Complement Activation In ELISA
Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för medicinska vetenskaper, Klinisk kemi.
Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för medicinska vetenskaper, Klinisk kemi.
2002 (engelsk)Inngår i: Upsala Journal of Medical Sciences, ISSN 0300-9734, E-ISSN 2000-1967, Vol. 106, nr 3, s. 189-195Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Antibodies derived from egg yolk offer many advantages over mammalian antibodies in several aspects. Chicken antibodies do not activate the human complement system and are sometimes a more suitable choice in designing solid-phase immunometric assays than mammalian antibodies. The material often recommended for immunological assays is serum. A freshly drawn serum sample contains an active complement system, which is inactivated during storage. Mammalian antibodies used in most immunological assays may activate the human complement system. Activated complement components will bind to the antibodies thereby partly block the antibody binding epitopes. We show that an active complement system in undiluted samples reduce the absorbance values by approximately 50 % when using goat antibodies but not when using chicken antibodies. This difference will cause erroneous test results that will vary depending on the handling of the samples. Chicken antibodies can be used to eliminate this interference problem.

sted, utgiver, år, opplag, sider
2002. Vol. 106, nr 3, s. 189-195
HSV kategori
Identifikatorer
URN: urn:nbn:se:uu:diva-89637PubMedID: 12166511OAI: oai:DiVA.org:uu-89637DiVA, id: diva2:161293
Tilgjengelig fra: 2002-02-11 Laget: 2002-02-11 Sist oppdatert: 2017-12-14bibliografisk kontrollert
Inngår i avhandling
1. Avian IgY antibody: In vitro and in vivo
Åpne denne publikasjonen i ny fane eller vindu >>Avian IgY antibody: In vitro and in vivo
2002 (engelsk)Doktoravhandling, med artikler (Annet vitenskapelig)
Abstract [en]

Immunoglobulin Y (IgY) is the major antibody found in eggs from chicken (Gallus domesticus). IgY can be used as an alternative to mammalian antibodies normally used in research, and its use in immunotherapy has recently been proposed. Compared to mammalian antibodies, IgY possesses several biochemical advantages and its simple purification from egg yolk prevents a stressful moment in animal handling, as no bleeding is necessary.

Small amount of antigen (1 mg) can be used to elicit an immune response in chickens and there are low intra-individual differences regarding antibody concentration found in yolk. By studying two chicken breeds and their cross, a genetic correlation was shown regarding the IgY concentration, which implies a possibility by breeding to increase IgY concentrations. By using IgY instead of goat antibody as capture antibody in ELISA, it is possible reduce interferences by complement activation. After oral administration of IgY to healthy volunteers, IgY activity was present in saliva 8 hours later, indicating a protective effect. This effect has been studied in an open clinical trial with cystic fibrosis patients. Specific IgY against Pseudomonas aeruginosa given orally prolongs the time of intermittent colonization by six months, decrease the number of positive colonizations and might be a useful complement to antibiotic treatment. Immunoglobulin therapy may diminish the development of antibiotic resistant microorganisms. The use of immunoglobulin therapy broadens the arsenal available to combat pathogens in medicine and IgY is a promising candidate, both as an alternative to antibiotics and as a useful tool in research and diagnostics.

sted, utgiver, år, opplag, sider
Uppsala: Acta Universitatis Upsaliensis, 2002. s. 53
Serie
Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine, ISSN 0282-7476 ; 1119
Emneord
Medical sciences, Antigen production, chicken, cystic fibrosis, egg, ELISA, IgY, immunotherapy, Pseudomonas aeruginosa, yolk, MEDICIN OCH VÅRD
HSV kategori
Forskningsprogram
klinisk kemi
Identifikatorer
urn:nbn:se:uu:diva-1710 (URN)91-554-5227-2 (ISBN)
Disputas
2002-03-06, Rosénssalen, Ing 95, Akademiska sjukhuset, Uppsala, 09:15
Opponent
Tilgjengelig fra: 2002-02-11 Laget: 2002-02-11bibliografisk kontrollert

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