A new transgenic reporter line reveals expression of protocadherin 9 at a cellular level within the zebrafish central nervous systemShow others and affiliations
2022 (English)In: Gene Expression Patterns, ISSN 1567-133X, E-ISSN 1872-7298, Vol. 44, article id 119246Article in journal (Refereed) Published
Abstract [en]
The wiring of neuronal networks is far from understood. One outstanding question is how neurons of different types link up to form subnetworks within the greater context. Cadherins have been suggested to create an inclusion code where interconnected neurons express the same subtypes. Here, we have used a CRISPR/Cas9 knock-in approach to generate a transgenic zebrafish reporter line for protocadherin 9 (pcdh9), which is predominantly expressed within the central nervous system. Expression of eGFP was detected in subsets of neurons in the cerebellum, retina and spinal cord, in both larvae and juveniles. A closer characterization of the spinal locomotor network revealed that a portion of distinct classes of both excitatory and inhibitory interneurons, as well as motor neurons, expressed pcdh9. This transgenic line could thus be used to test the cadherin network hypothesis, through electrophysiological characterization of eGFP positive cells, to show if these are synaptically connected and form a discrete network within the spinal cord.
Place, publisher, year, edition, pages
Elsevier BV Elsevier, 2022. Vol. 44, article id 119246
National Category
Neurosciences
Identifiers
URN: urn:nbn:se:uu:diva-476168DOI: 10.1016/j.gep.2022.119246ISI: 000797915400002PubMedID: 35427788OAI: oai:DiVA.org:uu-476168DiVA, id: diva2:1666927
Funder
Swedish Research Council, 2020-00943Swedish Research Council, 26004Swedish Foundation for Strategic ResearchKjell and Marta Beijer FoundationHarald and Greta Jeansson FoundationCarl Tryggers foundation The Swedish Brain FoundationMagnus Bergvall FoundationThe Royal Swedish Academy of SciencesÅke Wiberg FoundationOlle Engkvists stiftelseRagnar Söderbergs stiftelse2022-06-092022-06-092024-01-15Bibliographically approved