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Tissue-based diagnosis of systemic amyloidosis: Experience of the informal diagnostic center at Uppsala University Hospital
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology. Uppsala Univ Hosp, Clin Pathol, Uppsala, Sweden..
Uppsala Univ Hosp, Clin Pathol, Uppsala, Sweden.;Uppsala Univ, Dept Genet Immunol & Pathol, Uppsala, Sweden..
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology. Uppsala Univ Hosp, Clin Pathol, Uppsala, Sweden..
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology.
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2022 (English)In: Upsala Journal of Medical Sciences, ISSN 0300-9734, E-ISSN 2000-1967, Vol. 127, no 1, article id e8913Article in journal (Refereed) Epub ahead of print
Abstract [en]

Diagnosis of systemic amyloidosis is a clinical challenge and usually relies on a tissue biopsy. We have developed diagnostic methods based on the presence of amyloid deposits in abdominal subcutaneous fat tissue. This tissue is also used to determine the biochemical type of amyloidosis, performed by western blot and immunohistochemical analyses with the aid of in-house developed rabbit antisera and mouse monoclonal antibodies. Mass spectrometric methods are under development for selected cases. The diagnostic outcome for 2018-2020 was studied. During this period, we obtained 1,562 biopsies, of which 1,397 were unfixed subcutaneous fat tissue with varying degrees of suspicion of systemic amyloidosis. Of these, 440 contained amyloid deposits. The biochemical nature of the amyloid was determined by western blot analysis in 319 specimens and by immunohistochemistry in further 51 cases.

Place, publisher, year, edition, pages
Upsala Medical Society , 2022. Vol. 127, no 1, article id e8913
Keywords [en]
Amyloidosis, systemic, monoclonal antibodies, subcutaneous fat tissue, biopsy
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy) Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-488926DOI: 10.48101/ujms.v127.8913ISI: 000879745200001PubMedID: 36337276OAI: oai:DiVA.org:uu-488926DiVA, id: diva2:1714471
Available from: 2022-11-29 Created: 2022-11-29 Last updated: 2022-11-29Bibliographically approved

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Thelander, UlrikaZancanaro, AliceWestermark, GunillaWestermark, Per

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Department of Immunology, Genetics and PathologyDepartment of Medical Cell BiologyNeurooncology and neurodegeneration
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Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)Cell and Molecular Biology

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