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A full-length infectious cDNA clone of a dsRNA totivirus-like virus
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Molecular biophysics.ORCID iD: 0000-0002-6445-2095
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Molecular biophysics.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Molecular biophysics.ORCID iD: 0000-0002-4858-1196
2022 (English)In: Virology, ISSN 0042-6822, E-ISSN 1096-0341, Vol. 576, p. 127-133Article in journal (Refereed) Published
Abstract [en]

Totivirus-like viruses are a group of non-segmented double-stranded (ds)RNA viruses with two open reading frames, which were recently discovered and provisionally assigned to the Totiviridae family. Unlike yeast and protozoan Totiviridae viruses, these totivirus-like viruses infect a diverse spectrum of metazoan hosts and currently have enormous impacts on fisheries and agriculture. We developed the first infectious full-length cDNA clone of a totivirus-like virus, the Omono River virus (OmRV), and produced infectious particles using an RNA -transcript-based method. Compared with the parent wild-type particles from nature, the infectious-cloning OmRV particles have presented strong cytopathic effects, infectivity and similar morphology. Thus far, the established system is one of the few reported systems for generating a non-segmented dsRNA virus cDNA clone.

Place, publisher, year, edition, pages
Elsevier, 2022. Vol. 576, p. 127-133
Keywords [en]
dsRNA virus, Totivirus, OmRV, Mosquito, Infectious clone, Reverse genetics
National Category
Microbiology
Identifiers
URN: urn:nbn:se:uu:diva-490903DOI: 10.1016/j.virol.2022.09.010ISI: 000888431800001PubMedID: 36242926OAI: oai:DiVA.org:uu-490903DiVA, id: diva2:1720392
Funder
Swedish Research Council, 2018- 03387Swedish Research Council Formas, 2018-00421The Royal Swedish Academy of Sciences, BS2018-0053Available from: 2022-12-19 Created: 2022-12-19 Last updated: 2024-02-14Bibliographically approved
In thesis
1. Structural Decorations in Viruses: Unraveling Acquired Functional Structures in Icosahedral RNA Virus Capsids
Open this publication in new window or tab >>Structural Decorations in Viruses: Unraveling Acquired Functional Structures in Icosahedral RNA Virus Capsids
2024 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Viruses have a profound impact worldwide, posing challenges to animal welfare, agriculture, human health, and the ecosystem. This thesis examines the realm of non-enveloped icosahedral double-stranded (ds)RNA and single-stranded (ss)RNA viruses through three studies. In Paper I, we employed a reverse genetics approach to generate recombinant dsRNA totivirus-like viruses—which negatively impact fisheries and the economy—unraveling the intricate relationships between viral genes and life cycles. Our reverse genetic method has proven essential for generating infectious totivirus-like virus particles, allowing for a nuanced exploration of viral behaviors. Understanding these behaviors has the potential to help in developing effective virus control approaches. In Paper II, we elucidated the previously unknown capsid structure, uncovering the intriguing acquired features of a dsRNA megabirnavirus—Rosellinia necatrix megabirnavirus 1-W779 (RnMBV1)—through cryogenic electron microscopy single-particle analysis. RnMBV1, a fungal virus, has potential applications in controlling white root rot, a plant disease that causes substantial economic losses. Insights into this viral structural information can enhance our ability to leverage this fungal virus for economic and agricultural benefits. In Paper III, we obtained the capsid atomic models of a Marnaviridae ssRNA virus: Chaetoceros socialis forma radians RNA virus 1. Additionally, we generated a structure-based phylogeny using viral protein structures predicted by AlphaFold2; this was done to enhance our understanding of algal virus-host specificity. As harmful algal blooms (HABs) pose global threats to ecology and the economy, Chaetoceros algae have emerged as a contributing factor. Certain Marnaviridae viruses exhibit specific infection patterns in Chaetoceros, thereby influencing the occurrence and mitigation of HABs. Studies on Marnaviridae viruses collectively provide insights into the interactions between algal viruses and their hosts, paving the way for utilizing marine algal viruses to address HAB-related challenges. Together, our functional and structural analyses will contribute to a broader understanding of both dsRNA and ssRNA viruses, their behaviors, and their potential applications in addressing economic, agricultural, ecological, and healthcare issues.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2024. p. 69
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 2364
Keywords
Viruses, RNA viruses, capsid, structure, cryo-EM
National Category
Structural Biology
Research subject
Molecular Life Sciences
Identifiers
urn:nbn:se:uu:diva-523070 (URN)978-91-513-2035-9 (ISBN)
Public defence
2024-04-09, room A1:111a, BMC, Husargatan 3, Uppsala, 13:15 (English)
Opponent
Supervisors
Available from: 2024-03-08 Created: 2024-02-13 Last updated: 2024-03-08Bibliographically approved

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Wang, Hande Matos Filipe, DiogoOkamoto, Kenta

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