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Frontotemporal dementia in a large Swedish family is caused by a progranulin null mutation
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Public Health and Caring Sciences, Geriatrics. (Molekylär geriatrik)
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Public Health and Caring Sciences, Geriatrics. (Molekylär geriatrik)
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Genetics and Pathology.
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2009 (English)In: Neurogenetics, ISSN 1364-6745, E-ISSN 1364-6753, Vol. 10, no 1, p. 27-34Article in journal (Refereed) Published
Abstract [en]

Mutations in the progranulin (PGRN) gene have recently been identified in families with frontotemporal lobar degeneration and ubiquitin-positive brain inclusions linked to chromosome 17q21. We have previously described a Swedish family displaying frontotemporal dementia with rapid progression and linkage to chromosome 17q21. In this study, we performed an extended clinical and neuropathological investigation of affected members of the family and a genetic analysis of the PGRN gene. There was a large variation of the initial presenting symptoms in this family, but common clinical features were non-fluent aphasia and loss of spontaneous speech as well as personality and behavioural changes. Mean age at onset was 54 years with disease duration of close to 4 years. Neuropathological examination revealed frontotemporal neurodegeneration with ubiquitin and TAR DNA binding protein-43 immunoreactive intraneuronal inclusions. Mutation screening of the PGRN gene identified a 1 bp deletion in exon 1 causing a frameshift of the coding sequence and introducing a premature termination codon in exon 2 (Gly35GlufsX19). Analysis of PGRN messenger RNA (mRNA) levels revealed a considerable decrease in lymphoblasts from mutation carriers and fragment size separation, and sequence analysis confirmed that the mutated mRNA allele was almost absent in these samples. In conclusion, the PGRN Gly35fs mutation causes frontotemporal dementia with variable clinical presentation in a large Swedish family, most likely through nonsense-mediated decay of mutant PGRN mRNA and resulting haploinsufficiency.

Place, publisher, year, edition, pages
2009. Vol. 10, no 1, p. 27-34
Keywords [en]
Frontotemporal lobar degeneration, Frontotemporal dementia, Progranulinn, Ubiquitin, TDP-43
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-98042DOI: 10.1007/s10048-008-0155-zISI: 000262652300005PubMedID: 18855025OAI: oai:DiVA.org:uu-98042DiVA, id: diva2:173208
Available from: 2009-02-13 Created: 2009-02-13 Last updated: 2022-01-28Bibliographically approved
In thesis
1. Molecular Mechanisms of Frontotemporal Lobar Degeneration
Open this publication in new window or tab >>Molecular Mechanisms of Frontotemporal Lobar Degeneration
2009 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The aim of this thesis was to identify genetic factors involved in frontotemporal lobar degeneration (FTLD), a neurodegenerative disorder clinically characterised by a progressive change in personality, behaviour and language. FTLD is a genetically complex disorder and a positive family history is found in up to 40% of the cases.

In 10-20% of the familial cases the disease can be explained by mutations in the gene encoding the microtubule associated protein tau (MAPT). In the first study we describe the clinical and neuropathological features of a Finnish family with FTLD caused by a mutation in MAPT. We also provide evidence that the pathogenic mechanism of this mutation is through altered splicing of MAPT transcripts.

Recently, mutations in the gene encoding progranulin (PGRN) were identified as a major cause of FTLD. In the second study we describe a Swedish family with FTLD caused by a frameshift mutation in PGRN. We provide a clinical and neuropathological description of the family, as well as evidence that the pathogenicity of this mutation is through nonsense-mediated decay of the mutant mRNA transcripts and PGRN haploinsufficiency.

In the third study we describe a novel PGRN splice site mutation and a previously described PGRN frameshift mutation, found in a mutation screen of 51 FTLD patients. We describe the clinical and neuropathological characteristics of the mutation carriers and demonstrate that haploinsufficiency is the pathogenic mechanism of the two mutations.

In the fourth study we investigate the prevalence of PGRN and MAPT gene dosage alterations in 39 patients with FTLD. No gene dosage alterations were identified, indicating that variations in copy number of the PGRN and MAPT genes are not a common cause of disease, at least not in this FTLD patient collection.

Place, publisher, year, edition, pages
Uppsala: Universitetsbiblioteket, 2009. p. 52
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine, ISSN 1651-6206 ; 418
Keywords
Frontotemporal lobar degeneration, Frontotemporal dementia, Tau, Progranulin, Alternative splicing, Nonsense-mediated decay, Haploinsufficiency
Identifiers
urn:nbn:se:uu:diva-9550 (URN)978-91-554-7405-8 (ISBN)
Public defence
2009-03-06, Rudbecksalen, Rudbecklaboratoriet, Dag Hammarskjölds väg 20, Uppsala, 09:15
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Supervisors
Available from: 2009-02-13 Created: 2009-02-13Bibliographically approved

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Brundin, RoseMarieIngvast, SofieGiedraitis, VilmantasIngelsson, Martin

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Brundin, RoseMarieKalimo, HannuIngvast, SofieBlom, Elin SGiedraitis, VilmantasIngelsson, MartinLannfelt, LarsBasun, HansGlaser, Anna
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GeriatricsDepartment of Surgical SciencesDepartment of Genetics and Pathology
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