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Live Detection of Neural Progenitors and Glioblastoma Cells by an Oligothiophene Derivative
Karolinska Inst, Dept Neurosci, S-17177 Stockholm, Sweden..
Karolinska Inst, Dept Neurosci, S-17177 Stockholm, Sweden..
Linköping Univ, Dept Chem, IFM, S-58183 Linköping, Sweden..ORCID iD: 0000-0001-5222-9307
Linköping Univ, Dept Chem, IFM, S-58183 Linköping, Sweden..
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2023 (English)In: ACS Applied Bio Materials, E-ISSN 2576-6422, Vol. 6, no 9, p. 3790-3797Article in journal (Refereed) Published
Abstract [en]

There is an urgent need for simple and non-invasive identification of live neural stem/progenitor cells (NSPCs) in the developing and adult brain as well as in disease, such as in brain tumors, due to the potential clinical importance in prognosis, diagnosis, and treatment of diseases of the nervous system. Here, we report a luminescent conjugated oligothiophene (LCO), named p-HTMI, for non-invasive and non-amplified real-time detection of live human patient-derived glioblastoma (GBM) stem cell-like cells and NSPCs. While p-HTMI stained only a small fraction of other cell types investigated, the mere addition of p-HTMI to the cell culture resulted in efficient detection of NSPCs or GBM cells from rodents and humans within minutes. p-HTMI is functionalized with a methylated imidazole moiety resembling the side chain of histidine/histamine, and non-methylated analogues were not functional. Cell sorting experiments of human GBM cells demonstrated that p-HTMI labeled the same cell population as CD271, a proposed marker for stem cell-like cells and rapidly migrating cells in glioblastoma. Our results suggest that the LCO p-HTMI is a versatile tool for immediate and selective detection of neural and glioma stem and progenitor cells.

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2023. Vol. 6, no 9, p. 3790-3797
Keywords [en]
bioelectronics, progenitor, brain tumor, methylation, p75NTR
National Category
Cancer and Oncology Neurosciences Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-519795DOI: 10.1021/acsabm.3c00447ISI: 001064987700001PubMedID: 37647213OAI: oai:DiVA.org:uu-519795DiVA, id: diva2:1825936
Funder
Swedish Cancer SocietyKnut and Alice Wallenberg FoundationSwedish Foundation for Strategic ResearchEU, European Research CouncilVinnovaSwedish Research CouncilAvailable from: 2024-01-10 Created: 2024-01-10 Last updated: 2024-01-10Bibliographically approved

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Nelander, SvenWestermark, BengtUhrbom, LeneForsberg Nilsson, Karin

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Aslund, Andreas K. O.Nelander, SvenWestermark, BengtUhrbom, LeneForsberg Nilsson, Karin
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