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Noninvasive acoustic cell trapping in a microfluidic perfusion system for online bioassays
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
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2007 (English)In: Analytical Chemistry, ISSN 0003-2700, E-ISSN 1520-6882, Vol. 79, no 7, p. 2984-2991Article in journal (Refereed) Published
Abstract [en]

Techniques for manipulating, separating, and trapping particles and cells are highly desired in today's bioanalytical and biomedical field. The microfluidic chip-based acoustic noncontact trapping method earlier developed within the group now provides a flexible platform for performing cell- and particle-based assays in continuous flow microsystems. An acoustic standing wave is generated in etched glass channels (600x61 microm2) by miniature ultrasonic transducers (550x550x200 microm3). Particles or cells passing the transducer will be retained and levitated in the center of the channel without any contact with the channel walls. The maximum trapping force was calculated to be 430+/-135 pN by measuring the drag force exerted on a single particle levitated in the standing wave. The temperature increase in the channel was characterized by fluorescence measurements using rhodamine B, and levels of moderate temperature increase were noted. Neural stem cells were acoustically trapped and shown to be viable after 15 min. Further evidence of the mild cell handling conditions was demonstrated as yeast cells were successfully cultured for 6 h in the acoustic trap while being perfused by the cell medium at a flowrate of 1 microL/min. The acoustic microchip method facilitates trapping of single cells as well as larger cell clusters. The noncontact mode of cell handling is especially important when studies on nonadherent cells are performed, e.g., stem cells, yeast cells, or blood cells, as mechanical stress and surface interaction are minimized. The demonstrated acoustic trapping of cells and particles enables cell- or particle-based bioassays to be performed in a continuous flow format.

Place, publisher, year, edition, pages
2007. Vol. 79, no 7, p. 2984-2991
Keywords [en]
Perfusion, Blood, Rhodamine, Fluorescence spectrometry, Transducer, Glass, Acoustic wave, Continuous flow method, Chemical analysis, Acoustic method, System on a chip, Biochemical analysis, Biological indicator, Bioassay, On line, Microfluidics, Trapping
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:uu:diva-100675DOI: 10.1021/ac061576vISI: 000245304300047PubMedID: 17313183OAI: oai:DiVA.org:uu-100675DiVA, id: diva2:210771
Available from: 2009-04-06 Created: 2009-04-05 Last updated: 2017-12-13Bibliographically approved
In thesis
1. Acoustic Manipulation of Particles and Fluids in Microfluidic Systems
Open this publication in new window or tab >>Acoustic Manipulation of Particles and Fluids in Microfluidic Systems
2009 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The downscaling and integration of biomedical analyses onto a single chip offers several advantages in speed, cost, parallelism and de-centralization. Acoustic radiation forces are attractive to use in these applications since they are strong, long-range and gentle. Lab-on-a-chip operations such as cell trapping, particle fluorescence activated cell sorting, fluid mixing and particle sorting performed by acoustic radiation forces are exploited in this thesis. Two different platforms are designed, manufactured and evaluated.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2009. p. 81
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 641
National Category
Fluid Mechanics and Acoustics
Research subject
Engineering Science with specialization in Microsystems Technology
Identifiers
urn:nbn:se:uu:diva-100758 (URN)978-91-554-7513-0 (ISBN)
Public defence
2009-05-15, Seigbahnsalen, Ångström laboratories, Regementsv. 1, Uppsala, 13:15 (English)
Opponent
Supervisors
Available from: 2009-04-24 Created: 2009-04-07 Last updated: 2010-07-19Bibliographically approved

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Johansson, Stefan

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