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On-chip fluorescence activated cell sorting by an integrated miniaturized ultrasonic transducer
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Micro Structural Technology.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Micro Structural Technology. Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Micro Structural Technology.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Micro Structural Technology.
2009 (English)In: Analytical Chemistry, ISSN 0003-2700, E-ISSN 1520-6882, Vol. 81, no 13, p. 5188-5196Article in journal (Refereed) Published
Abstract [en]

An acoustic microfluidic system for miniaturized fluorescence-activated   cell sorting (mu FACS) is presented. By excitation of a miniaturized   piezoelectric transducer at 10 MHz in the microfluidic channel bottom, an acoustic standing wave is formed in the channel. The acoustic   radiation force acting on a density interface causes fluidic movement, and the particles or cells on either side of the fluid interface are displaced in a direction perpendicular to the standing wave direction. The small size of the transducer enables individual manipulation of   cells passing the transducer surface. At constant transducer activation   the system was shown to accomplish up to 700 mu m sideways displacement   of 10 mu m beads in a 1 mm wide channel. This is much larger than if   utilizing the acoustic radiation force acting directly on particles, where the limitation in maximum displacement is between a node and an antinode which at 10 MHz is 35 mu m. In the automatic sorting setup,   the system was demonstrated to successfully sort single cells of E-GFP expressing beta-cells.

Place, publisher, year, edition, pages
2009. Vol. 81, no 13, p. 5188-5196
National Category
Chemical Sciences Engineering and Technology
Identifiers
URN: urn:nbn:se:uu:diva-100677DOI: 10.1021/ac802681rISI: 000267609500014OAI: oai:DiVA.org:uu-100677DiVA, id: diva2:210773
Available from: 2009-04-08 Created: 2009-04-05 Last updated: 2017-12-13Bibliographically approved
In thesis
1. Acoustic Manipulation of Particles and Fluids in Microfluidic Systems
Open this publication in new window or tab >>Acoustic Manipulation of Particles and Fluids in Microfluidic Systems
2009 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The downscaling and integration of biomedical analyses onto a single chip offers several advantages in speed, cost, parallelism and de-centralization. Acoustic radiation forces are attractive to use in these applications since they are strong, long-range and gentle. Lab-on-a-chip operations such as cell trapping, particle fluorescence activated cell sorting, fluid mixing and particle sorting performed by acoustic radiation forces are exploited in this thesis. Two different platforms are designed, manufactured and evaluated.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2009. p. 81
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 641
National Category
Fluid Mechanics and Acoustics
Research subject
Engineering Science with specialization in Microsystems Technology
Identifiers
urn:nbn:se:uu:diva-100758 (URN)978-91-554-7513-0 (ISBN)
Public defence
2009-05-15, Seigbahnsalen, Ångström laboratories, Regementsv. 1, Uppsala, 13:15 (English)
Opponent
Supervisors
Available from: 2009-04-24 Created: 2009-04-07 Last updated: 2010-07-19Bibliographically approved

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Nikolajeff, FredrikJohansson, StefanThorslund, Sara

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