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Acoustic Manipulation of Particles and Fluids in Microfluidic Systems
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Micro Structural Technology.
2009 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The downscaling and integration of biomedical analyses onto a single chip offers several advantages in speed, cost, parallelism and de-centralization. Acoustic radiation forces are attractive to use in these applications since they are strong, long-range and gentle. Lab-on-a-chip operations such as cell trapping, particle fluorescence activated cell sorting, fluid mixing and particle sorting performed by acoustic radiation forces are exploited in this thesis. Two different platforms are designed, manufactured and evaluated.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis , 2009. , p. 81
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 641
National Category
Fluid Mechanics and Acoustics
Research subject
Engineering Science with specialization in Microsystems Technology
Identifiers
URN: urn:nbn:se:uu:diva-100758ISBN: 978-91-554-7513-0 (print)OAI: oai:DiVA.org:uu-100758DiVA, id: diva2:211240
Public defence
2009-05-15, Seigbahnsalen, Ångström laboratories, Regementsv. 1, Uppsala, 13:15 (English)
Opponent
Supervisors
Available from: 2009-04-24 Created: 2009-04-07 Last updated: 2010-07-19Bibliographically approved
List of papers
1. An Evaluation of the Temperature Increase from PZT Micro-Transducers for Acoustic Trapping
Open this publication in new window or tab >>An Evaluation of the Temperature Increase from PZT Micro-Transducers for Acoustic Trapping
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2005 (English)In: 2005 IEEE Ultrasonics Symposium, 2005, p. 1614-1617Conference paper, Published paper (Refereed)
Identifiers
urn:nbn:se:uu:diva-100674 (URN)0-7803-9382-1 (ISBN)
Available from: 2009-04-06 Created: 2009-04-05 Last updated: 2009-04-06Bibliographically approved
2. Noninvasive acoustic cell trapping in a microfluidic perfusion system for online bioassays
Open this publication in new window or tab >>Noninvasive acoustic cell trapping in a microfluidic perfusion system for online bioassays
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2007 (English)In: Analytical Chemistry, ISSN 0003-2700, E-ISSN 1520-6882, Vol. 79, no 7, p. 2984-2991Article in journal (Refereed) Published
Abstract [en]

Techniques for manipulating, separating, and trapping particles and cells are highly desired in today's bioanalytical and biomedical field. The microfluidic chip-based acoustic noncontact trapping method earlier developed within the group now provides a flexible platform for performing cell- and particle-based assays in continuous flow microsystems. An acoustic standing wave is generated in etched glass channels (600x61 microm2) by miniature ultrasonic transducers (550x550x200 microm3). Particles or cells passing the transducer will be retained and levitated in the center of the channel without any contact with the channel walls. The maximum trapping force was calculated to be 430+/-135 pN by measuring the drag force exerted on a single particle levitated in the standing wave. The temperature increase in the channel was characterized by fluorescence measurements using rhodamine B, and levels of moderate temperature increase were noted. Neural stem cells were acoustically trapped and shown to be viable after 15 min. Further evidence of the mild cell handling conditions was demonstrated as yeast cells were successfully cultured for 6 h in the acoustic trap while being perfused by the cell medium at a flowrate of 1 microL/min. The acoustic microchip method facilitates trapping of single cells as well as larger cell clusters. The noncontact mode of cell handling is especially important when studies on nonadherent cells are performed, e.g., stem cells, yeast cells, or blood cells, as mechanical stress and surface interaction are minimized. The demonstrated acoustic trapping of cells and particles enables cell- or particle-based bioassays to be performed in a continuous flow format.

Keywords
Perfusion, Blood, Rhodamine, Fluorescence spectrometry, Transducer, Glass, Acoustic wave, Continuous flow method, Chemical analysis, Acoustic method, System on a chip, Biochemical analysis, Biological indicator, Bioassay, On line, Microfluidics, Trapping
National Category
Engineering and Technology
Identifiers
urn:nbn:se:uu:diva-100675 (URN)10.1021/ac061576v (DOI)000245304300047 ()17313183 (PubMedID)
Available from: 2009-04-06 Created: 2009-04-05 Last updated: 2017-12-13Bibliographically approved
3. Temperature control and resonance mode analysis of an acoustic trap for μTAS
Open this publication in new window or tab >>Temperature control and resonance mode analysis of an acoustic trap for μTAS
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(English)Article in journal (Refereed) Submitted
Identifiers
urn:nbn:se:uu:diva-100678 (URN)
Available from: 2009-04-08 Created: 2009-04-05 Last updated: 2009-04-08Bibliographically approved
4.
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5. On-chip fluorescence activated cell sorting by an integrated miniaturized ultrasonic transducer
Open this publication in new window or tab >>On-chip fluorescence activated cell sorting by an integrated miniaturized ultrasonic transducer
2009 (English)In: Analytical Chemistry, ISSN 0003-2700, E-ISSN 1520-6882, Vol. 81, no 13, p. 5188-5196Article in journal (Refereed) Published
Abstract [en]

An acoustic microfluidic system for miniaturized fluorescence-activated   cell sorting (mu FACS) is presented. By excitation of a miniaturized   piezoelectric transducer at 10 MHz in the microfluidic channel bottom, an acoustic standing wave is formed in the channel. The acoustic   radiation force acting on a density interface causes fluidic movement, and the particles or cells on either side of the fluid interface are displaced in a direction perpendicular to the standing wave direction. The small size of the transducer enables individual manipulation of   cells passing the transducer surface. At constant transducer activation   the system was shown to accomplish up to 700 mu m sideways displacement   of 10 mu m beads in a 1 mm wide channel. This is much larger than if   utilizing the acoustic radiation force acting directly on particles, where the limitation in maximum displacement is between a node and an antinode which at 10 MHz is 35 mu m. In the automatic sorting setup,   the system was demonstrated to successfully sort single cells of E-GFP expressing beta-cells.

National Category
Chemical Sciences Engineering and Technology
Identifiers
urn:nbn:se:uu:diva-100677 (URN)10.1021/ac802681r (DOI)000267609500014 ()
Available from: 2009-04-08 Created: 2009-04-05 Last updated: 2017-12-13Bibliographically approved
6. Surface Acoustic Wave-induced particle sorting with node-position flexibility
Open this publication in new window or tab >>Surface Acoustic Wave-induced particle sorting with node-position flexibility
(English)In: Lab on a Chip, ISSN 1473-0197, E-ISSN 1473-0189Article in journal (Refereed) Submitted
Identifiers
urn:nbn:se:uu:diva-100812 (URN)
Available from: 2009-04-07 Created: 2009-04-07 Last updated: 2017-12-13Bibliographically approved
7. Surface Acoustic Wave-induced particle manipulation in a glass microfluidic channel
Open this publication in new window or tab >>Surface Acoustic Wave-induced particle manipulation in a glass microfluidic channel
(English)In: Journal of Micromechanics and Microengineering, ISSN 0960-1317, E-ISSN 1361-6439Article in journal (Refereed) Submitted
Keywords
surface avoustic wave, manipulation, acoustic
Identifiers
urn:nbn:se:uu:diva-100904 (URN)
Available from: 2009-04-10 Created: 2009-04-10 Last updated: 2017-12-13Bibliographically approved
8. Acoustic manipulation of sub-micrometer particles by interface waves in microfluidic channels
Open this publication in new window or tab >>Acoustic manipulation of sub-micrometer particles by interface waves in microfluidic channels
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(English)In: Lab on a Chip, ISSN 1473-0197, E-ISSN 1473-0189Article in journal (Refereed) Submitted
Identifiers
urn:nbn:se:uu:diva-100905 (URN)
Available from: 2009-04-10 Created: 2009-04-10 Last updated: 2017-12-13Bibliographically approved

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