uu.seUppsala universitets publikasjoner
Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Surface-Confined Photopolymerization of pH-Responsive Acrylamide/Acrylate Brushes on Polymer Thin Films
Department of Chemistry, KTH.
Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Tekniska sektionen, Institutionen för teknikvetenskaper, Fasta tillståndets elektronik. (Thin Films Group)
IFM, Linköping University.
Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Fysiska sektionen, Institutionen för fysik och materialvetenskap.
Vise andre og tillknytning
2008 (engelsk)Inngår i: Langmuir, ISSN 0743-7463, E-ISSN 1520-5827, Vol. 24, nr 14, s. 7559-7564Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Dynamic acrylamide/acrylate polymeric brushes were synthesized at gold-plated quartz crystal surfaces. The crystals were initially coated with polystyrene-type thin films, derivatized with photolabile iniferter groups, and subsequently subjected to photoinitiated polymerization in acrylamide/acrylate monomer feeds. This surface-confined polymerizationmethod enabled direct photocontrol over the polymerization, as followed by increased frequency responses of the crystal oscillations in a quartz crystal microbalance (QCM). The produced polymer layers were also found to be highlysensitive to external acid/base stimuli. Large oscillation frequency shifts were detected when the brushes were exposedto buffer solutions of different pH. The dynamic behavior of the resulting polymeric brushes was evaluated, and theextent of expansion and contraction of the films was monitored by the QCM setup in situ in real time. The resultingresponses were rapid, and the effects were fully reversible. Low pH resulted in full contractions of the films, whereashigher pH yielded maximal expansion in order to minimize repulsion around the charged acrylate centers. The surfacesalso proved to be very robust because the responsiveness was reproducible over many cycles of repeated expansionand contraction. Using ellipsometry, copolymer layers were estimated to be ∼220 nm in a collapsed state and ∼340nm in the expanded state, effectively increasing the thickness of the film by 55%.

sted, utgiver, år, opplag, sider
2008. Vol. 24, nr 14, s. 7559-7564
Emneord [en]
Surface grafting, polymer brushes, hydrogels, sensor surfaces, transfer radical polymerization
HSV kategori
Forskningsprogram
Ytbioteknik
Identifikatorer
URN: urn:nbn:se:uu:diva-107234DOI: 10.1021/la800700hISI: 000257468300072PubMedID: 18563922OAI: oai:DiVA.org:uu-107234DiVA, id: diva2:228374
Tilgjengelig fra: 2009-07-30 Laget: 2009-07-30 Sist oppdatert: 2017-12-13bibliografisk kontrollert
Inngår i avhandling
1. Development of Electroacoustic Sensors for Biomolecular Interaction Analysis
Åpne denne publikasjonen i ny fane eller vindu >>Development of Electroacoustic Sensors for Biomolecular Interaction Analysis
2009 (engelsk)Doktoravhandling, med artikler (Annet vitenskapelig)
Abstract [en]

Biomolecular interaction analysis to determine the kinetics and affinity between interacting partners is important for the fundamental understanding of biology, as well as for the development of new pharmaceutical substances. A quartz crystal microbalance instrument suitable for kinetics and affinity analyses of interaction events was developed. The functionality of the sensor system was demonstrated by development of an assay for relative affinity determination of lectin-carbohydrate interactions.

Sensor surfaces allowing for effective immobilization of one interacting partner is a key functionality of a biosensor. Here, three different surfaces and immobilization methods were studied. First, optimized preparation conditions for sensor surfaces based on carboxyl-terminated self assembled monolayers were developed and were demonstrated to provide highly functional biosensor surfaces with low non-specific binding. Second, a method allowing for immobilization of very acidic biomolecules based on the use of an electric field was developed and evaluated. The electric field made it possible to immobilize the highly acidic C-peptide on a carboxylated surface. Third, a method for antibody immobilization on a carboxyl surface was optimized and the influence of immobilization pH on the immobilization level and antigen binding capacity was thoroughly assessed. The method showed high reproducibility for a set of antibodies and allowed for antibody immobilization also at low pH.

Three broadly different strategies to increase the sensitivity of electroacoustic sensors were explored. A QCM sensor with small resonator electrodes and reduced flow cell dimensions was demonstrated to improve the mass transport rate to the sensor surface. The use of polymers on QCM sensor surfaces to enhance the sensor response was shown to increase the response of an antibody-antigen model system more than ten-fold. Moreover, the application of high frequency thin film bulk acoustic resonators for biosensing was evaluated with respect to sensing range from the surface. The linear detection range of the thin film resonator was determined to be more than sufficient for biosensor applications involving, for instance, antibody-antigen interactions. Finally, a setup for combined frequency and resistance measurements was developed and was found to provide time resolved data suitable for kinetics determination.

sted, utgiver, år, opplag, sider
Uppsala: Acta Universitatis Upsaliensis, 2009. s. 68
Serie
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 658
Emneord
biosensor, protein interactions, kinetics, affinity, QCM, quartz crystal microbalance, piezoelectric resonators, dissipation, motional resistance
HSV kategori
Forskningsprogram
Teknisk fysik med inriktning mot mikrosystemteknik
Identifikatorer
urn:nbn:se:uu:diva-107211 (URN)978-91-554-7572-7 (ISBN)
Disputas
2009-09-11, Å80101, Ångströmlaboratoriet, Lägerhyddsvägen 1, Uppsala, 09:30 (engelsk)
Opponent
Veileder
Prosjekter
wisenet
Tilgjengelig fra: 2009-08-20 Laget: 2009-07-29 Sist oppdatert: 2014-11-25bibliografisk kontrollert

Open Access i DiVA

Fulltekst mangler i DiVA

Andre lenker

Forlagets fulltekstPubMed

Personposter BETA

Anderson, Henrik

Søk i DiVA

Av forfatter/redaktør
Anderson, Henrik
Av organisasjonen
I samme tidsskrift
Langmuir

Søk utenfor DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric

doi
pubmed
urn-nbn
Totalt: 1014 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf