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Microfluidic high viability neural cell separation using viscoelastically tuned hydrodynamic spreading
Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Tekniska sektionen, Institutionen för teknikvetenskaper, Mikrostrukturteknik.
Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Tekniska sektionen, Institutionen för teknikvetenskaper, Mikrostrukturteknik.
Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap.
Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap.
2008 (engelsk)Inngår i: Biomedical microdevices (Print), ISSN 1387-2176, E-ISSN 1572-8781, Vol. 10, nr 5, s. 631-638Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

A high viability microfluidic cell separation technique of high throughput was demonstrated based on size difference continuous mode hydrodynamic spreading with viscoelastic tuning. Using water with fluorescent dye as sample fluid and in parallel introducing as elution a viscoelastic biocompatible polymer solution of alginic sodium, the spreading behavior was investigated at different polymer concentrations and flow rates. Particle separation was studied in the same detail for 9.9 mu m and 1.9 mu m latex beads. Using buffered aqueous solutions and further surface treatments to protect from cell adhesion, separation between neuron cells and glial cells from rat's spine cord was demonstrated and compared to the separation of latex particles of 20 and 4.6 mu m sizes. High relative viability (above 90%) of neural cells was demonstrated compared the reference cells of the same batch.

sted, utgiver, år, opplag, sider
2008. Vol. 10, nr 5, s. 631-638
Emneord [en]
microfluidics, viscoelastic, neural cell, separation
HSV kategori
Identifikatorer
URN: urn:nbn:se:uu:diva-109131DOI: 10.1007/s10544-008-9174-7ISI: 000258581700004OAI: oai:DiVA.org:uu-109131DiVA, id: diva2:248941
Tilgjengelig fra: 2009-10-09 Laget: 2009-10-09 Sist oppdatert: 2017-12-13bibliografisk kontrollert

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