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Extraction of Antioxidants from Spruce (Picea abies) Bark using Eco-Friendly Solvents
Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för fysikalisk och analytisk kemi, Analytisk kemi.
Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för biokemi och organisk kemi.
Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - BMC.
Department of Natural Sciences, Engineering and Mathematics, Mid Sweden University.
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2012 (Engelska)Ingår i: Phytochemical Analysis, ISSN 0958-0344, E-ISSN 1099-1565, Vol. 23, nr 1, s. 1-11Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Introduction-Antioxidants are known to avert oxidation processes and they are found in trees and other plant materials. Tree bark is a major waste product from paper pulp industries; hence it is worthwhile to develop an extraction technique to extract the antioxidants.

Objective- To develop a fast and environmentally sustainable extraction technique for the extraction of antioxidants from bark of spruce (Picea abies) and also to identify the extracted antioxidants that are abundant in spruce bark.

Methodology- A screening experiment that involved three different techniques, was conducted to determine the best technique to extract antioxidants.The antioxidant capacity of the extracts was determined with DPPH (2,2-diphenyl-2’-picrylhydrazyl) assay. Pressurised fluid extraction (PFE) turned out to be the best technique and a response surface design was therefore utilised to optimise PFE. Furthermore, NMR and HPLC-DAD-MS/MS were applied to identify the extracted antioxidants.

Results- PFE using water and ethanol as solvent at 160 and 180°C, respectively, gave extracts of the highest antioxidant capacity. Stilbene glucosides such as isorhapontin, piceid and astringin were identified in the extracts.

Conclusion-The study has shown that PFE is a fast and environmentally sustainable technique, using water and ethanol as solvent for the extraction of antioxidants from spruce bark.

Ort, förlag, år, upplaga, sidor
Wiley-Blackwell, 2012. Vol. 23, nr 1, s. 1-11
Nyckelord [en]
Accelerated solvent extraction, antioxidant, DPPH, ethanol, Picea abies, pressurised fluid extraction, supercritical fluid extraction, water
Nationell ämneskategori
Annan medicinsk grundvetenskap
Forskningsämne
Analytisk kemi
Identifikatorer
URN: urn:nbn:se:uu:diva-133267DOI: 10.1002/pca.1316ISI: 000298260100001OAI: oai:DiVA.org:uu-133267DiVA, id: diva2:360759
Forskningsfinansiär
VetenskapsrådetTillgänglig från: 2010-11-04 Skapad: 2010-11-04 Senast uppdaterad: 2018-01-12Bibliografiskt granskad
Ingår i avhandling
1. Pressurised Fluid Extraction of Bioactive Species in Tree Barks: Analysis using Hyphenated Electrochemical Mass Spectrometric Detection
Öppna denna publikation i ny flik eller fönster >>Pressurised Fluid Extraction of Bioactive Species in Tree Barks: Analysis using Hyphenated Electrochemical Mass Spectrometric Detection
2010 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Abstract [en]

Analytical chemistry has developed throughout time to meet current needs. At present, the interest in biorefinery is growing, due to environmental awareness and the depletion of fossil resources. Biomass from agricultural and forestry industries has proven to be excellent raw material for different processes. Biorefinering valuable species such as bioactive species from biomass, without compromising the primary process of the biomass is highly desirable. Pressurised fluid extraction (PFE) using water and ethanol as a solvent was developed for extracting betulin from birch (Betula pendula) bark. Apart from betulin, stilbene glucosides such as astringin, isorhapontin and picied were also extracted from spruce (Picea abies) using PFE. PFE is an advanced technique that extracts at temperatures above the solvent’s atmospheric boiling point. The applied pressure in PFE is mainly to maintain the liquid state of the extraction solvent. Parameters such as type of solvent, temperature, and time affect the extraction selectivity and efficiency. Therefore it is necessary to comprehend these parameters in order to optimise extraction. The DPPH (1,1-diphenyl-2-picrylhydrazyl) assay was used to determine the antioxidant capacity and activity of the obtained bioactive species. The results showed high antioxidant capacity in bioactive species that were extracted at an elevated temperature, 180°C. Extraction and degradation occur simultaneously during the extraction. Hence, it is crucial to separate these two processes in order to obtain the actual value.

An online hyphenated system of chromatographic separation electrochemical mass spectrometric detection was developed (LC-DAD-ECD-MS/MS). The electrochemical detector facilitates real-time monitoring of the antioxidant capacity and activity of each antioxidant and its oxidation products. This developed LC-DAD-ECD-MS/MS method enabled rapid screening of antioxidants and created a fingerprint map for their oxidation products. Characterisation and molecular elucidation of bioactive species were also performed. Degradation of bioactive species was investigated with the said online system and birch bark extract was compared with birch bark extracts that were hydrothermally treated. The obtained results showed some degradation of antioxidants at 180°C.

In summary, the aim of this thesis was to develop analytical methods integrated with sustainable chemistry for extraction of bioactive species in biomass from the forestry industry. A novel online system using selective and sensitive detectors such as diode-array, electrochemical, and tandem mass spectrometry was developed to rapidly determine the antioxidant capacity and activity of antioxidants. Furthermore, tandem mass spectrometry enables identification of unknown bioactive species without the need of reference samples.

Ort, förlag, år, upplaga, sidor
Uppsala: Acta Universitatis Upsaliensis, 2010. s. 82
Serie
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 789
Nyckelord
Pressurised fluid extraction, antioxidants, DPPH, water, ethanol, antioxidant activity, antioxidant capacity, electrochemial detection, mass spektrometry
Nationell ämneskategori
Annan medicinsk grundvetenskap
Forskningsämne
Analytisk kemi
Identifikatorer
urn:nbn:se:uu:diva-133264 (URN)978-91-554-7951-0 (ISBN)
Disputation
2010-12-17, B22, Husargatan 3, Uppsala, 10:15 (Engelska)
Opponent
Handledare
Anmärkning
Felaktigt tryckt som Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology 719Tillgänglig från: 2010-11-26 Skapad: 2010-11-04 Senast uppdaterad: 2018-01-12Bibliografiskt granskad

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Engman, LarsSjöberg, Per J. R

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