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Replication-biased genome organisation in the crenarchaeon Sulfolobus
Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för ekologi och evolution. Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för ekologi och genetik, Limnologi.
Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för evolution, genomik och systematik, Molekylär evolution.
Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för evolution, genomik och systematik, Molekylär evolution.
Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för evolution, genomik och systematik, Molekylär evolution. Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för cell- och molekylärbiologi, Mikrobiologi.
Vise andre og tillknytning
2010 (engelsk)Inngår i: BMC Genomics, ISSN 1471-2164, E-ISSN 1471-2164, Vol. 11, s. 454-Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Background: Species of the crenarchaeon Sulfolobus harbour three replication origins in their single circular chromosome that are synchronously initiated during replication. Results: We demonstrate that global gene expression in two Sulfolobus species is highly biased, such that early replicating genome regions are more highly expressed at all three origins. The bias by far exceeds what would be anticipated by gene dosage effects alone. In addition, early replicating regions are denser in archaeal core genes (enriched in essential functions), display lower intergenic distances, and are devoid of mobile genetic elements. Conclusion: The strong replication-biased structuring of the Sulfolobus chromosome implies that the multiple replication origins serve purposes other than simply shortening the time required for replication. The higher-level chromosomal organisation could be of importance for minimizing the impact of DNA damage, and may also be linked to transcriptional regulation.

sted, utgiver, år, opplag, sider
2010. Vol. 11, s. 454-
HSV kategori
Identifikatorer
URN: urn:nbn:se:uu:diva-134173DOI: 10.1186/1471-2164-11-454ISI: 000282787800003OAI: oai:DiVA.org:uu-134173DiVA, id: diva2:371788
Tilgjengelig fra: 2010-11-22 Laget: 2010-11-22 Sist oppdatert: 2017-12-12bibliografisk kontrollert
Inngår i avhandling
1. Unique Solutions to Universal Problems: Studies of the Archaeal Cell
Åpne denne publikasjonen i ny fane eller vindu >>Unique Solutions to Universal Problems: Studies of the Archaeal Cell
2012 (engelsk)Doktoravhandling, med artikler (Annet vitenskapelig)
Abstract [en]

Archaea is one of the three domains of life and studies of archaeal biology are important for understanding of life in extreme environments, fundamental biogeochemical processes, the origin of life, the eukaryotic cell and their own, unique biology. This thesis presents four studies of the archaeal cell, using the extremophilic Sulfolobus and ocean living Nitrosopumilus as model systems.

Cell division in crenarchaea is shown to be carried out by a previously unknown system named Cdv (cell division). The system shares homology with the eukaryotic ESCRT-III system which is used for membrane reorganization during vesicle formation, viral release and cytokinesis. Organisms of the phylum Thaumarchaeota also use the Cdv system, despite also carrying genes for the euryarchaeal and bacterial cell division system FtsZ.

The thaumarchaeal cell cycle is demonstrated to be dominated by the prereplicative and replicative stage, in contrasts to the crenarchaeal cell cycle where the cell at the majority of the time resides in the postreplicative stage. The replication rate is remarkably low and closer to what is measured for eukaryotes than other archaea.

The gene organization of Sulfolobus is significantly associated with the three origins of replication. The surrounding regions are dense with genes of high importance for the organisms such as highly transcribed genes, genes with known function in fundamental cellular processes and conserved archaeal genes. The overall gene density is elevated and transposons are underrepresented.

The archaeal virus SIRV2 displays a lytic life style where the host cell at the final stage of infection is disrupted for release of new virus particles. The remarkable pyramid-like structure VAP (virus associated pyramids), that is formed independently of the virus particle, is used for cell lysis.

The research presented in this thesis describes unique features of the archaeal cell and influences our understanding of the entire tree of life.

sted, utgiver, år, opplag, sider
Uppsala: Acta Universitatis Upsaliensis, 2012. s. 72
Serie
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 888
Emneord
Archaea, Cdv, Cell cycle, Cell division, Cell lysis, Crenarchaea, ESCRT-III, Flow cytometry, Microarray, Microscopy, Nitrosopumilus, SIRV2, Sulfolobus, Thaumarchaea, Transcription, VAP, Virus
HSV kategori
Forskningsprogram
Biologi med inriktning mot molekylär evolution
Identifikatorer
urn:nbn:se:uu:diva-162886 (URN)978-91-554-8244-2 (ISBN)
Disputas
2012-02-03, C4:301, Biomedicinskt Centrum, Husargatan 3; BMC, Uppsala, 13:15 (engelsk)
Opponent
Veileder
Tilgjengelig fra: 2012-01-10 Laget: 2011-12-05 Sist oppdatert: 2012-01-16

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