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Sensitive detection of genes by sandwich hybridization and time-resolved fluorometry
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1987 (engelsk)Inngår i: Molecular and Cellular Probes, ISSN 1044-7431, E-ISSN 1095-9327, Vol. 1, nr 2, s. 159-168Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Europium has been used as a non-radioactive marker in immunoassays as this metal can be detected with high sensitivity by time-resolved fluorometry. In this work streptavidin labeled with europium was used to detect biotinylated probes in a sandwich nucleic-acid hybridization assay with microtitration strips as the solid phase. pBR 322 plasmids were detected with a sensitivity of 4 × 105 molecules. As the sample is added in solution in sandwich hybridization, fast and simple sample pre-treatment can be used without encountering background problems. The method was applied to test bacterial samples of uropathogenic Escherichia coli strains for the presence of the β-lactamase gene.

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1987. Vol. 1, nr 2, s. 159-168
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URN: urn:nbn:se:uu:diva-169911DOI: 10.1016/0890-8508(87)90024-7PubMedID: 3331173OAI: oai:DiVA.org:uu-169911DiVA, id: diva2:508017
Tilgjengelig fra: 2012-03-07 Laget: 2012-03-07 Sist oppdatert: 2017-12-07bibliografisk kontrollert

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