uu.seUppsala universitets publikationer
Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Human Myocardial Protein Pattern Reveals Cardiac Diseases
Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - BMC, Analytisk kemi.
Bruker Daltonik GmbH, 28359 Bremen, Germany.
Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för medicinska vetenskaper, Klinisk fysiologi.
Bruker Daltonik GmbH, 28359 Bremen, Germany.
Visa övriga samt affilieringar
2012 (Engelska)Ingår i: International Journal of Proteomics, ISSN 2090-2174, Vol. 2012, s. 342659-Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Proteomic profiles of myocardial tissue in two different etiologies of heart failure were investigated using high performance liquid chromatography (HPLC)/Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS). Right atrial appendages from 10 patients with hemodynamically significant isolated aortic valve disease and from 10 patients with isolated symptomatic coronary heart disease were collected during elective cardiac surgery. As presented in an earlier study by our group (Baykut et al., 2006), both disease forms showed clearly different pattern distribution characteristics. Interesting enough, the classification patterns could be used for correctly sorting unknown test samples in their correct categories. However, in order to fully exploit and also validate these findings there is a definite need for unambiguous identification of the differences between different etiologies at molecular level. In this study, samples representative for the aortic valve disease and coronary heart disease were prepared, tryptically digested, and analyzed using an FT-ICR MS that allowed collision-induced dissociation (CID) of selected classifier masses. By using the fragment spectra, proteins were identified by database searches. For comparison and further validation, classifier masses were also fragmented and analyzed using HPLC-/Matrix-assisted laser desorption ionization (MALDI) time-offlight/time-of-flight (TOF/TOF) mass spectrometry. Desmin and lumican precursor were examples of proteins found in aortic samples at higher abundances than in coronary samples. Similarly, adenylate kinase isoenzyme was found in coronary samples at a higher abundance. The described methodology could also be feasible in search for specific biomarkers in plasma or serum for diagnostic purposes.

Ort, förlag, år, upplaga, sidor
Hindawi Publishing Corporation, 2012. Vol. 2012, s. 342659-
Nationell ämneskategori
Medicin och hälsovetenskap Naturvetenskap
Identifikatorer
URN: urn:nbn:se:uu:diva-179821DOI: 10.1155/2012/342659OAI: oai:DiVA.org:uu-179821DiVA, id: diva2:546466
Forskningsfinansiär
VetenskapsrådetTillgänglig från: 2012-08-23 Skapad: 2012-08-23 Senast uppdaterad: 2012-08-23Bibliografiskt granskad

Open Access i DiVA

fulltext(4538 kB)550 nedladdningar
Filinformation
Filnamn FULLTEXT01.pdfFilstorlek 4538 kBChecksumma SHA-512
78711d1a3d30916b29e80c72e35b3c6e8d24b74d6307cb9b12b31eca958718e61ad664ebec4b7e93f905412ee907ed1985ed5194d66023a1ada2dd0d0a9c1fcb
Typ fulltextMimetyp application/pdf

Övriga länkar

Förlagets fulltext

Personposter BETA

Bergquist, JonasBergquist, Maria

Sök vidare i DiVA

Av författaren/redaktören
Bergquist, JonasBergquist, Maria
Av organisationen
Analytisk kemiKlinisk fysiologi
Medicin och hälsovetenskapNaturvetenskap

Sök vidare utanför DiVA

GoogleGoogle Scholar
Totalt: 550 nedladdningar
Antalet nedladdningar är summan av nedladdningar för alla fulltexter. Det kan inkludera t.ex tidigare versioner som nu inte längre är tillgängliga.

doi
urn-nbn

Altmetricpoäng

doi
urn-nbn
Totalt: 1143 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf