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Influence of Flap Prefabrication on Seeding of Subcutaneously Injected Mesenchymal Stem Cells in Microvascular Beds in Rats
Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för kirurgiska vetenskaper, Plastikkirurgi.
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2014 (Engelska)Ingår i: Annals of Plastic Surgery, ISSN 0148-7043, E-ISSN 1536-3708, Vol. 73, nr 2, s. 234-238Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Background: In this article, the authors investigated whether the prefabrication of an autologous pedicled flap by isolation from the surrounding with artificial skin substitutes would increase mesenchymal stem cell (MSC) seeding. Methods: Mesenchymal stem cells were isolated from human umbilical cords and were cultured and characterized by fluorescence-activated cell sorting. Oxacarbocyanine and its green fluorescence emission were used to label the MSCs population. Sixteen adult Wistar rats were randomized in 4 groups (n = 4 animals per group). In group 1, a prefabricated groin flap (GF) with skin substitutes was harvested without cell injection; in group 2, 1 million MSCs were injected subcutaneously in the area corresponding to the GF without flap harvesting; in Group 3, a prefabricated GF with skin substitutes was harvested and 1 million MSCs were injected subcutaneously; and in Group 4, a prefabricated GF with skin substitutes was harvested and 2 million MSCs were injected subcutaneously. All procedures were performed bilaterally in each animal. Animals were sacrificed 2 weeks after the surgery. Flap viability was then assessed by clinical inspection and histology, and seeding of MSCs was observed. Results: All flaps survived 2 weeks after the surgery. Oxacarbocyanine-labeled cells were found in all prefabricated flaps injected (Groups 3 and 4) in higher number in comparison with the group where subcutaneous injection without flap harvesting was performed (Group 2). This difference was statistically significant (P < 0.05). Conclusions: Prefabricated skin flaps with skin substitutes may provide a useful vehicle for the implantation of MSCs to serve as an autologous microvascular bioscaffold.

Ort, förlag, år, upplaga, sidor
2014. Vol. 73, nr 2, s. 234-238
Nyckelord [en]
flap prefabrication, mesenchymal stem cells, skin substitutes, seeding, tissue engineering
Nationell ämneskategori
Kirurgi
Identifikatorer
URN: urn:nbn:se:uu:diva-230953DOI: 10.1097/SAP.0000000000000074ISI: 000340144200025OAI: oai:DiVA.org:uu-230953DiVA, id: diva2:743210
Tillgänglig från: 2014-09-03 Skapad: 2014-09-01 Senast uppdaterad: 2017-12-05Bibliografiskt granskad

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Rodriguez-Lorenzo, Andres

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