Observing Growth and Division of Large Numbers of Individual Bacteria by Image Analysis
2004 (English)In: Applied and Environmental Microbiology, Vol. 70, no 2, p. 675-678Article in journal (Refereed) Published
Abstract [en]
We describe a method that enabled us to observe large numbers of individual bacterial cells during a long period of cell growth and proliferation. We designed a flow chamber in which the cells attached to a transparent solid surface. The flow chamber was mounted on a microscope equipped with a digital camera. The shear force of the flow removed the daughter cells, making it possible to monitor the consecutive divisions of a single cell. In this way, kinetic parameters and their distributions, as well as some physiological characteristics of the bacteria, could be analyzed based on more than 1,000 single-cell observations. The method which we developed enabled us to study the history effect on the distribution of the lag times of single cells.
Place, publisher, year, edition, pages
2004. Vol. 70, no 2, p. 675-678
Identifiers
URN: urn:nbn:se:uu:diva-47058OAI: oai:DiVA.org:uu-47058DiVA, id: diva2:74965
2007-02-142007-02-142011-01-12