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Ubiquinone-10 alters mechanical properties and increases stability of phospholipid membranes
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Analytical Chemistry.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Analytical Chemistry.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Analytical Chemistry.
2015 (English)In: Biochimica et Biophysica Acta - Biomembranes, ISSN 0005-2736, E-ISSN 1879-2642, Vol. 1848, no 10, p. 2233-2243Article in journal (Refereed) Published
Abstract [en]

Abstract Ubiquinone-10 is mostly known for its role as an electron and proton carrier in aerobic cellular respiration and its function as a powerful antioxidant. Accumulating evidence suggest, however, that this well studied membrane component could have several other important functions in living cells. The current study reports on a previously undocumented ability of ubiquinone-10 to modulate the mechanical strength and permeability of lipid membranes. Investigations of DPH fluorescence anisotropy, spontaneous and surfactant induced leakage of carboxyfluorescein, and interactions with hydrophobic and hydrophilic surfaces were used to probe the effects caused by inclusion of ubiquinone-10 in the membrane of phospholipid liposomes. The results show that ubiquinone in concentrations as low as 2 mol.% increases the lipid packing order and condenses the membrane. The altered physicochemical properties result in a slower rate of release of hydrophilic components, and render the membrane more resistant towards rupture. As judged from comparative experiments using the polyisoprenoid alcohol solanesol, the quinone moiety is essential for the membrane stabilizing effects to occur. Our findings imply that the influence of ubiquinone-10 on the permeability and mechanical properties of phospholipid membranes is similar to that of cholesterol. The reported data indicate, however, that the molecular mechanisms are different in the two cases.

Place, publisher, year, edition, pages
2015. Vol. 1848, no 10, p. 2233-2243
Keywords [en]
Coenzyme Q10, Liposomes, Leakage, Membrane stability, Solanesol
National Category
Chemical Sciences
Identifiers
URN: urn:nbn:se:uu:diva-253627DOI: 10.1016/j.bbamem.2015.05.002ISI: 000362153400032OAI: oai:DiVA.org:uu-253627DiVA, id: diva2:815269
Funder
Swedish Research Council, 621-2011-3524Swedish Cancer Society, CAN20111504Available from: 2015-05-29 Created: 2015-05-29 Last updated: 2018-09-23Bibliographically approved
In thesis
1. Effects of Ubiquinone-10 on the Stability and Mechanical Properties of Lipid Membranes
Open this publication in new window or tab >>Effects of Ubiquinone-10 on the Stability and Mechanical Properties of Lipid Membranes
2018 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Ubiquinones are a group of fat-soluble molecules present in many biological membranes. The most abundant version in humans, ubiquinone-10 (Q10), plays an important role in the mitochondrial respiration chain and also functions as a powerful antioxidant. Accumulating evidence suggests that Q10 also could have other functions in the membrane. The aim of this thesis has been to explore Q10’s possible role as a membrane stabilizer.

To investigate the potential effect of Q10 in membranes, liposomes with compositions of biological relevance were used as models systems. In lipid systems mimicking that of the inner membrane of the mitochondria, Q10 was found to lower the membrane’s permeability to hydrophilic solutes, render the membrane more resistant to rupturing and promote membrane lipid order. In models mimicking the plasma membrane of E.coli, Q10 was observed to decrease the water permeability and increase the elastic resistance against membrane deformation during osmotic shock. All in all, the results suggest a general membrane stabilizing effect of Q10. The results indicate, however, that the extent of, as well as the mechanisms behind, the membrane stabilizing effects of Q10 vary depending on the membrane lipid composition. Part of the reason for this can likely be traced back to differences in the intermembrane location of Q10.

Supplementary experiments, which facilitated the investigations of Q10 membrane effects, revealed that the choice of cuvette material was of importance for liposome leakage experiments with fluorescent hydrophilic dyes. The results of these experiments highlight the need to take liposome-cuvette interactions into account when planning and evaluating spectroscopic studies involving liposomes.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2018. p. 64
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 1725
Keywords
ubiquinone-10, lipid bilayers, membrane stabilizer, liposome permeability, osmotic shock, cuvettes, solanesol
National Category
Physical Chemistry
Research subject
Chemistry with specialization in Physical Chemistry
Identifiers
urn:nbn:se:uu:diva-361361 (URN)978-91-513-0450-2 (ISBN)
Public defence
2018-11-09, BMC A1:111a, BMC, Husargatan 3, Uppsala, 09:00 (English)
Opponent
Supervisors
Available from: 2018-10-19 Created: 2018-09-23 Last updated: 2018-11-19

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Agmo Hernández, VíctorEriksson, Emma K.Edwards, Katarina

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