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Surface Coating of Pancreatic Islets With Neural Crest Stem Cells Improves Engraftment and Function After Intraportal Transplantation
Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för medicinsk cellbiologi. Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för medicinska vetenskaper, Klinisk diabetologi och metabolism.
Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för medicinsk cellbiologi. Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap.
Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap.
Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för medicinsk cellbiologi. Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för medicinska vetenskaper, Transplantation och regenerativ medicin.
2015 (engelsk)Inngår i: Cell Transplantation, ISSN 0963-6897, E-ISSN 1555-3892, Vol. 24, nr 11, s. 2263-2272Artikkel i tidsskrift (Fagfellevurdert) Published
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Abstract [en]

The present study aimed to develop techniques for surface coating of islets with neural crest stem cells (NCSCs) in order to enable cotransplantation to the clinically used liver site and then investigate engraftment and function intraportally of such bioengineered islets. Mouse islets were coated during incubation with enhanced green fluorescent protein (EGFP)-expressing mouse NCSCs and transplanted into the portal vein to cure diabetic mice. An intravenous glucose tolerance test was performed at 1 month posttransplantation. Islet grafts were retrieved and evaluated for vascular density, nerves, and glial cells. NCSCs expressed a vast number of key angiogenic and neurotrophic factors. Mice transplanted with NCSC-bioengineered islets responded better to the glucose load than recipient mice with control islets. NCSCs remained present in the vicinity or had often migrated into the NCSC-coated islets, and an improved islet graft reinnervation and revascularization was observed. Transplanted NCSCs differentiated into both glial and neural cells in the islet grafts. We conclude that bioengineering of islets with NCSCs for intraportal transplantation provides a possibility to improve islet engraftment and function. Pending successful establishment of protocols for expansion of NCSCs from, for example, human skin or bone marrow, this strategy may be applied to clinical islet transplantation.

sted, utgiver, år, opplag, sider
2015. Vol. 24, nr 11, s. 2263-2272
Emneord [en]
Islet transplantation, Stem cells, Neural progenitors, Engraftment, Revascularization, Reinnervation
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Identifikatorer
URN: urn:nbn:se:uu:diva-269293DOI: 10.3727/096368915X686184ISI: 000364353300008PubMedID: 25581301OAI: oai:DiVA.org:uu-269293DiVA, id: diva2:882614
Forskningsfinansiär
Swedish Research Council, 20716Swedish Research Council, 55X-15043Novo NordiskSwedish Diabetes AssociationSwedish Child Diabetes FoundationNovo NordiskAFA InsuranceSwedish Society of MedicineMagnus Bergvall FoundationTilgjengelig fra: 2015-12-15 Laget: 2015-12-15 Sist oppdatert: 2017-12-01bibliografisk kontrollert

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