uu.seUppsala universitets publikationer
Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
The Cannabinoid Receptor-1 Is an Imaging Biomarker of Brown Adipose Tissue
Univ Turku, Turku PET Ctr, FI-20521 Turku, Finland.;Abo Akad Univ, Dept Biosci, Turku, Finland..
Univ Turku, Turku PET Ctr, FI-20521 Turku, Finland..
Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för medicinsk cellbiologi.
Univ Turku, Turku PET Ctr, FI-20521 Turku, Finland.;Univ Turku, Dept Psychiat, FI-20521 Turku, Finland..
Visa övriga samt affilieringar
2015 (Engelska)Ingår i: Journal of Nuclear Medicine, ISSN 0161-5505, E-ISSN 1535-5667, Vol. 56, nr 12, s. 1937-1941Artikel i tidskrift (Refereegranskat) Published
Resurstyp
Text
Abstract [en]

Recently, the existence of significant deposits of brown adipose tissue (BAT) in human adults was confirmed. Its role in the human metabolism is unknown but could be substantial. Inhibition of the cannabinoid receptor-1 (CB1) by the antagonist rimonabant (SR141716) has been associated with activation of BAT thermogenesis and weight loss in mice and rats. The role of peripheral and central CB1 in the activation of BAT merits further investigation. Here we developed a technique for quantifying CB1 in BAT by PET. Methods: Sections of rat BAT and subcutaneous white adipose tissue (WAT) were stained for CB1 and uncoupling protein-1 by immunofluorescent staining. Binding of the radiolabeled CB1 antagonist (3R,5R)-5-(3-(18F-fluoromethoxy)phenyl)-3-(((R)-1-phenylethyl)amino)-1-(4-(trifluoromethyl)-phenyl)pyrrolidin-2-one (F-18-FMPEP-d(2)) to BAT in vivo and in vitro was assessed in rats by PET. Results: We found that CB1 was colocalized with uncoupling protein-1 in BAT, but neither protein was found in WAT. Binding of the radiotracer to BAT sections (but not WAT) in vitro was high and displaceable by pretreatment with rimonabant. Deposits of BAT in rats had significant binding of F-18-FMPEP-d(2) in vivo, indicating high CB1 density. WAT deposits were negative for F-18-FMPEP-d(2), consistent with the immunofluorescent staining and in vitro results. Conclusion: F-18-FMPEP-d(2) PET can quantify CB1 density noninvasively in vivo in rats. CB1 is therefore a promising surrogate imaging biomarker for assessing the presence of BAT deposits as well as for elucidating the mechanism of CB1 antagonist-mediated weight loss.

Ort, förlag, år, upplaga, sidor
2015. Vol. 56, nr 12, s. 1937-1941
Nyckelord [en]
brown adipose tissue, cannabinoid 1 receptor, F-18-FMPEP-d(2), positron emission tomography
Nationell ämneskategori
Radiologi och bildbehandling
Identifikatorer
URN: urn:nbn:se:uu:diva-270949DOI: 10.2967/jnumed.115.156422ISI: 000365724800022PubMedID: 26359260OAI: oai:DiVA.org:uu-270949DiVA, id: diva2:890885
Forskningsfinansiär
EU, FP7, Sjunde ramprogrammetTillgänglig från: 2016-01-05 Skapad: 2016-01-05 Senast uppdaterad: 2017-12-01Bibliografiskt granskad

Open Access i DiVA

Fulltext saknas i DiVA

Övriga länkar

Förlagets fulltextPubMed

Personposter BETA

Espes, Daniel

Sök vidare i DiVA

Av författaren/redaktören
Espes, Daniel
Av organisationen
Institutionen för medicinsk cellbiologi
I samma tidskrift
Journal of Nuclear Medicine
Radiologi och bildbehandling

Sök vidare utanför DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetricpoäng

doi
pubmed
urn-nbn
Totalt: 483 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf