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Mammary epithelial cell and adipocyte co-culture in a 3-D matrix: the first step towards tissue-engineered human breast tissue.
2001 (Engelska)Ingår i: Cells Tissues Organs, ISSN 1422-6405, E-ISSN 1422-6421, Vol. 169, nr 4Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Reconstruction of the female breast after cancer surgery is a demanding task where the methods used today suffer from several disadvantages. In the present study we have investigated the possibility to use tissue engineering methods to regenerate human autologous breast tissue. Human mammary epithelial cells and preadipocytes were derived from breast tissue biopsies from healthy women undergoing reduction mammoplasty, and the two celltypes were co-cultured with conventional cell culture methods as well as in 3-D matrices. The study shows that it is possible to harvest both human mammary epithelial cells and preadipocytes in a single session, propagate several subcultures, and that the cells maintain a normal intercellular distribution and growth-pattern when co-cultured in a 3-D collagen gel. We propose that growth and formation of a tissue closely resembling normal human breast tissue be readily obtained in the described in vitro cell culture set-up using basic tissue engineering principles. This concept may be of great importance in the development of new methods for reconstruction of the human breast.

Ort, förlag, år, upplaga, sidor
2001. Vol. 169, nr 4
Nationell ämneskategori
Kirurgi
Forskningsämne
Plastikkirurgi
Identifikatorer
URN: urn:nbn:se:uu:diva-286418DOI: 47903PubMedID: 11490115OAI: oai:DiVA.org:uu-286418DiVA, id: diva2:921510
Tillgänglig från: 2016-04-20 Skapad: 2016-04-20 Senast uppdaterad: 2017-11-30
Ingår i avhandling
1. In vitro and in vivo studies of tissue engineering in reconstructive plastic surgery
Öppna denna publikation i ny flik eller fönster >>In vitro and in vivo studies of tissue engineering in reconstructive plastic surgery
2005 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Ort, förlag, år, upplaga, sidor
Linköping: Unitryck, 2005. s. 75
Serie
Linköping University Medical Dissertations, ISSN 0345-0082 ; 905
Nationell ämneskategori
Kirurgi
Forskningsämne
Plastikkirurgi
Identifikatorer
urn:nbn:se:uu:diva-286421 (URN)91-85299-06-5 (ISBN)
Disputation
2005-09-02, Linköping, 14:43 (Engelska)
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Tillgänglig från: 2016-04-22 Skapad: 2016-04-20 Senast uppdaterad: 2016-04-22Bibliografiskt granskad

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