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Method verification for homocysteine and a sustainability study on glucose, homocysteine and lactate in different sampling tubes
Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för kvinnors och barns hälsa.
2016 (engelsk)Independent thesis Basic level (professional degree), 10 poäng / 15 hpOppgave
Abstract [en]

The pre-analytical phase is known for being the most important step in the laboratory process to reach reliable test results. If handling, transport or preparation of the sample is performed incorrectly the results can deviate from the true value. Today, sampling tubes contains various additives to stabilize concentration levels. The aim of this study was to test a new sampling tube containing fluoride/citrate for glucose, lactate and homocysteine. It was also of interest to evaluate the stability of those three analytes in lithium-heparin, sodium-fluoride/potassium oxalate and fluoride/citrate tubes. To perform the sustainability study, a method verification was done for homocysteine in plasma. The study was performed in a hospital laboratory on the routine instrument Roche Cobas 6000 analyzer. Blood was drawn from 20 patients and was analyzed at the hospital laboratory in Gävle. The blood samples were transported frozen to the laboratory in Hudiksvall and were used in the method verification. For the sustainability study, blood was drawn from 10 healthy volunteers in lithium-heparin, sodium-fluoride/potassium oxalate and fluoride/citrate tubes. The method verification was approved. The results showed that glucose was stable for up to 72 hours in Vacuette Glycaemia tube with fluoride/citrate and this tube also gave more accurate results. Lactate and homocysteine were also stable in fluoride/citrate, but needs further studies. All three analytes were more stable if the sample tubes were centrifuged as soon as possible after blood collection. Fluoride/citrate tubes were stable without centrifugation directly.

sted, utgiver, år, opplag, sider
2016. , s. 23
Emneord [en]
pre-analytical factors, fluoride citrate, Cobas 6000, lithium heparin, sodium fluoride/potassium oxalate
HSV kategori
Identifikatorer
URN: urn:nbn:se:uu:diva-296043OAI: oai:DiVA.org:uu-296043DiVA, id: diva2:935978
Fag / kurs
Chemistry
Utdanningsprogram
Biomedical Laboratory Science Programme; Biomedical Laboratory Science Programme
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Examiner
Tilgjengelig fra: 2017-09-04 Laget: 2016-06-13 Sist oppdatert: 2017-09-04bibliografisk kontrollert

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