Autoantibodies against 21-hydroxylase and side-chain cleavage enzyme in autoimmune Addison's disease are mainly immunoglobulin G1
2004 (English)In: European Journal of Endocrinology, ISSN 0804-4643, E-ISSN 1479-683X, Vol. 150, no 1, 49-56 p.Article in journal (Other academic) Published
OBJECTIVE: Immunoglobulin G (IgG) antibodies to the steroidogenic enzymes 21-hydroxylase (21OH) and side-chain cleavage enzyme (SCC) are important diagnostic markers for autoimmune Addison's disease and autoimmune polyendocrine syndromes (APS) types I and II. The characterization of autoantibody (IgG) subclasses may reveal information on how tIssue destruction takes place; therefore, IgG subtypes of anti-21OH and anti-SCC antibodies from sera of patients with Addison's disease, APS I and APS II were determined using recombinant 21OH and SCC. METHODS: SCC(51-521) and his-SCC(51-521) were expressed by pET-scc in the Escherichia coli strain BL21 Star (DE3) and inclusion bodies were purified. Full-length, human 21OH fused to an N-terminal 6x histidine affinity tag was expressed in insect cells by using the baculovirus expression system bac-to-bac. Western blots were used to investigate the IgG subtype(s) of the autoantibodies against 21OH and SCC in patients and healthy blood donors. RESULTS: All anti-SCC positive sera (n=10) contained autoantibodies of the IgG1 subclass, while four out of ten also contained IgG3. All anti-21OH positive sera (n=16) had autoantibodies exclusively against IgG1. Sera from 20 healthy subjects did not show any reactivity against 21OH or SCC. CONCLUSIONS: The finding of a predominating IgG1 response against 21OH and SCC may suggest that T helper (Th) cells of the Th1 subclass are involved in destruction of the adrenal cortex in patients with autoimmune Addison's disease.
Place, publisher, year, edition, pages
2004. Vol. 150, no 1, 49-56 p.
Addison Disease/*immunology, Antibody Specificity, Autoantibodies/blood/*immunology, Biological Markers, Cholesterol Side-Chain Cleavage Enzyme/genetics/*immunology, Gene Expression Regulation; Enzymologic, Humans, Immunoglobulin G/blood/immunology, Research Support; Non-U.S. Gov't, Steroid 21-Hydroxylase/genetics/*immunology, T-Lymphocytes/immunology
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-72169PubMedID: 14713279OAI: oai:DiVA.org:uu-72169DiVA: diva2:100080