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Analysis of antibody reactivity against cysteine sulfinic acid decarboxylase, a pyridoxal phosphate-dependent enzyme, in endocrine autoimmune disease
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences. (Autoimmuna sjukdomar (Kämpe))
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences. (Autoimmuna sjukdomar (Kämpe))
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2004 (English)In: Journal of Clinical Endocrinology and Metabolism, ISSN 0021-972X, E-ISSN 1945-7197, Vol. 89, no 4, 1636-1640 p.Article in journal (Other academic) Published
Abstract [en]

The structurally related group II pyridoxal phosphate (PLP)-dependent amino acid decarboxylases glutamic acid decarboxylase (GAD), aromatic L-amino acid decarboxylase (AADC), and histidine decarboxylase (HDC) are known autoantigens in endocrine disorders. We report, for the first time, the prevalence of serum autoantibody reactivity against cysteine sulfinic acid decarboxylase (CSAD), an enzyme that shares 50% amino acid identity with the 65- and 67-kDa isoforms of GAD (GAD-65 and GAD-67), in endocrine autoimmune disease. Three of 83 patients (3.6%) with autoimmune polyendocrine syndrome type 1 (APS1) were anti-CSAD positive in a radioimmunoprecipitation assay. Anti-CSAD antibodies cross-reacted with GAD-65, and the anti-CSAD-positive sera were also reactive with AADC and HDC. The low frequency of anti-CSAD reactivity is in striking contrast to the prevalence of antibodies against GAD-65, AADC, and HDC in APS1 patients, suggesting that different mechanisms control the immunological tolerance toward CSAD and the other group II decarboxylases. Moreover, CSAD may be a useful mold for the construction of recombinant chimerical antigens in attempts to map conformational epitopes on other group II PLP-dependent amino acid decarboxylases.

Place, publisher, year, edition, pages
2004. Vol. 89, no 4, 1636-1640 p.
Keyword [en]
Addison Disease/immunology, Aromatic-L-Amino-Acid Decarboxylases/immunology, Autoantibodies/*blood/immunology, Carboxy-Lyases/*immunology, Cross Reactions, Diabetes Mellitus; Type 1/immunology, Female, Glutamate Decarboxylase/immunology, Histidine Decarboxylase/immunology, Humans, Isoenzymes/immunology, Male, Polyendocrinopathies; Autoimmune/*immunology, Pyridoxal Phosphate/metabolism, Radioimmunoprecipitation Assay, Research Support; Non-U.S. Gov't
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Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-72223DOI: 10.1210/jc.2003-031161PubMedID: 15070923OAI: oai:DiVA.org:uu-72223DiVA: diva2:100134
Available from: 2005-09-13 Created: 2005-09-13 Last updated: 2010-05-19Bibliographically approved

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Sköldberg, FilipRorsman, FredrikPerheentupa, JaakkoGustafsson, JanKämpe, Olle

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