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Drug partition chromatography on immobilized porcine intestinal brush border membranes
Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Biochemistry.
Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Biochemistry.
2004 (English)In: Journal of chromatography A, Vol. 1031, 107-112 p.Article in journal (Refereed) Published
Abstract [en]

We immobilized porcine intestinal brush border membrane vesicles (BBMVs) for chromatographic analyses of drug partitioning into the membranes determined as Ks, the drug retention per phospholipid amount. For positive and neutral drugs Ks decreased day by day, whereas Ks for negative drugs increased marginally. Similar results on vesicle-lipid liposomes indicated a gradual loss of negative charge from the columns. The Ks values for positive drugs were higher than those for negative drugs with the same octanol/water partitioning or the same Ks on egg yolk phospholipid bilayers. Electrostatic interactions seem to be important for the partitioning of charged drugs into brush border membranes.

Place, publisher, year, edition, pages
2004. Vol. 1031, 107-112 p.
Keyword [en]
Liposomes, drug partitioning, immobilization, phospholipid bilayers, drugs
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-72731DOI: doi:10.1016/j.chroma.2003.11.063OAI: oai:DiVA.org:uu-72731DiVA: diva2:100642
Available from: 2005-05-27 Created: 2005-05-27 Last updated: 2011-01-12

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