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Ethanol weakens cytochalasin B binding to the GLUT1 glucose transporter and drug partitioning into lipid bilayers
Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Biochemistry.
Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Biochemistry.
Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Biochemistry.
2004 (English)In: Journal of chromatography A, Vol. 1031, 113-116 p.Article in journal (Refereed) Published
Abstract [en]

Ethanol weakens the specific interaction between the human red blood cell (RBC) glucose transporter GLUT1 and the inhibitor cytochalasin B (CB). The chromatographic retention volume of cytochalasin B on stationary phases consisting of GLUT1-containing membranes decreased with increasing ethanol concentration in the eluent. The apparent Kd values for the ethanol-GLUT1 interaction were 0.37, 0.45 and 0.64 M for red blood cells, red blood cell membrane vesicles and proteoliposomes, respectively, all much higher than the Kd values for D-glucose or cytochalasin B interaction with GLUT1. Ethanol also decreased the partitioning of cytochalasin B and drugs into phospholipid bilayers.

Place, publisher, year, edition, pages
2004. Vol. 1031, 113-116 p.
Keyword [en]
Immobilized biomembrane affinity chromatography, cytochalasin B, ethanol, glucose, GLUT1
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-72733DOI: doi:10.1016/j.chroma.2003.10.132OAI: oai:DiVA.org:uu-72733DiVA: diva2:100644
Available from: 2005-05-27 Created: 2005-05-27 Last updated: 2011-01-12

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Lagerquist Hägglund, Christine

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