Identification of the chicken MARCKS phosphorylation site specific for differentiating neurons as Ser 25 using a monoclonal antibody and mass spectrometry
2004 (English)In: Journal of Proteome Research, ISSN 1535-3893, E-ISSN 1535-3907, Vol. 3, no 1, 84-90 p.Article in journal (Refereed) Published
MARCKS is an actin-modulating protein that can be phosphorylated in multiple sites by PKC and proline-directed kinases. We have previously described a phosphorylated form of this protein specific for differentiating chick neurons, detected with mAb 3C3. Here, we show that this antibody binds to MARCKS only when it is phosphorylated at Ser 25. These and previous data provide hints for a possible answer to the question of why this ubiquitous protein seems to be essential only for neural development.
Place, publisher, year, edition, pages
2004. Vol. 3, no 1, 84-90 p.
Animals, Antibodies; Monoclonal, Binding Sites, Brain/cytology/embryology, Cell Differentiation, Chick Embryo, Intracellular Signaling Peptides and Proteins, Lipoproteins/*chemistry/metabolism/physiology, Membrane Proteins/*chemistry/metabolism/physiology, Neurons/chemistry/cytology, Phosphoproteins, Phosphorylation, Research Support; Non-U.S. Gov't, Retina/chemistry/cytology/embryology, Serine/immunology/metabolism, Spectrum Analysis; Mass/*methods
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-72989DOI: 10.1021/pr034066fPubMedID: 14998167OAI: oai:DiVA.org:uu-72989DiVA: diva2:100900