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Purification of a 75 kDa protein from the organelle matrix of human neutrophils and identification as N-acetylglucosamine-6-sulphatase
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences. (Inflammation)
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences. (Inflammation)
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Clinical Chemistry. (Biologisk struktur och funktion)
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
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2005 (English)In: Biochemical Journal, ISSN 0264-6021, Vol. 387, no Pt 3, 841-7 p.Article in journal (Refereed) Published
Abstract [en]

A 75 kDa protein was purified to homogeneity from granule extracts of normal human granulocytes using Sephadex G-75 chromatography, Mono-S cation exchange chromatography and chromatofocusing. The protein consisted of one chain with a molecular mass of 75 kDa, as determined by SDS/PAGE. Tryptic peptide analysis by MALDI-TOF (matrix-assisted laser-desorption ionization-time-of-flight) MS and sequence analysis by MS/MS identified the protein to be N-acetylglucosamine-6-sulphatase (EC 3.1.6.14). The identity of the protein was confirmed by demostrating enzymatic activity towards the substrate N-acetylglucosamine 6-sulphate. The enzyme was active over a broad pH range with an optimum of pH 7.0, and showed a Km value of 13.0 mM and a Vmax value of ~1.8 µM/min per mg. The enzyme also showed O-desulphation activity towards heparan sulphate-derived saccharides. Subcellular fractionation of neutrophil organelles showed the presence of enzymatic activity mainly in the same fractions as primary granules. Furthermore, PMA treatment of the neutrophils induced release of the enzyme, indicating its matrix protein nature. The presence of N-acetylglucosamine-6-sulphatase in human neutrophils implies that neutrophils may play a role in the modulation of cell surface molecules and extracellular matrix by O-desulphation.

Place, publisher, year, edition, pages
2005. Vol. 387, no Pt 3, 841-7 p.
Keyword [en]
granule, N-acetylglucosamine-6-sulphatase, neutrophil, O-desulphation, organelle matrix, release
National Category
Medical and Health Sciences
Research subject
Medical Biochemistry
Identifiers
URN: urn:nbn:se:uu:diva-73019DOI: 10.1042/BJ20041811PubMedID: 15595925OAI: oai:DiVA.org:uu-73019DiVA: diva2:100930
Available from: 2006-10-02 Created: 2006-10-02 Last updated: 2009-05-11Bibliographically approved

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Xu, ShengyuanZhao, LinshuLarsson, AndersVenge, Per

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