Effects of 2-methoxyestradiol on proliferation, apoptosis and PET-tracer uptake in human prostate cancer cell aggregates
2004 (English)In: Nuclear Medicine and Biology, ISSN 0969-8051, E-ISSN 1872-9614, Vol. 31, no 7, 867-874 p.Article in journal (Refereed) Published
The purpose of this study was to investigate the potential use of PET in vivo to record cytotoxic effects of 2-methoxyestradiol (2-ME), an endogenous metabolite of 17beta-estradiol. The anti-proliferative and pro-apoptotic effects of 2-ME on human prostate cancer cell (PC3) aggregates in vitro, were correlated with the uptake of fluoro-deoxy-D-glucose, FMAU and choline labelled with 18F, 11C, or 3H. 2-ME clearly reduced growth of PC3 aggregates and induced apoptosis in a dose-dependent manner. However, the uptake of the putative proliferation markers 11C-FMAU or 3H-choline failed to record the growth inhibitory effects of 2-ME on PC3 cell aggregates. The uptake of 18F-FDG was used as a marker for effects on cellular metabolism and also failed to show any dose-dependent effects in PC3 aggregates. The use of these PET-tracers in vivo is therefore not recommended in order to evaluate the cytotoxic effects of 2-ME on human prostate cancer cells.
Place, publisher, year, edition, pages
2004. Vol. 31, no 7, 867-874 p.
Antineoplastic Agents/administration & dosage, Apoptosis/*drug effects, Cell Aggregation/drug effects, Cell Line; Tumor, Cell Proliferation/*drug effects, Comparative Study, Dose-Response Relationship; Drug, Estradiol/*administration & dosage/*analogs & derivatives, Humans, Male, Neoplasm Staging/methods, Positron-Emission Tomography/*methods, Prostatic Neoplasms/drug therapy/*metabolism/pathology/*radionuclide imaging, Radioisotopes/*diagnostic use/*pharmacokinetics, Radiopharmaceuticals/diagnostic use/pharmacokinetics, Reproducibility of Results, Research Support; Non-U.S. Gov't, Sensitivity and Specificity
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-73054DOI: 10.1016/j.nucmedbio.2004.03.015PubMedID: 15464388OAI: oai:DiVA.org:uu-73054DiVA: diva2:100965