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Rho GTPases have diverse effects on the organization of the actin filament system
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Medicinska och farmaceutiska vetenskapsområdet, centrumbildningar mm , Ludwig Institute for Cancer Research.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Medicinska och farmaceutiska vetenskapsområdet, centrumbildningar mm , Ludwig Institute for Cancer Research.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences.
2004 (English)In: Biochemical Journal, ISSN 0264-6021, Vol. 377, no Pt 2, 327-337 p.Article in journal (Refereed) Published
Abstract [en]

The Rho GTPases are related to the Ras proto-oncogenes and consist of 22 family members. These proteins have important roles in regulating the organization of the actin filament system, and thereby the morphogenesis of vertebrate cells as well as their ability to migrate. In an effort to compare the effects of all members of the Rho GTPase family, active Rho GTPases were transfected into porcine aortic endothelial cells and the effects on the actin filament system were monitored. Cdc42, TCL (TC10-like), Rac1-Rac3 and RhoG induced the formation of lamellipodia, whereas Cdc42, Rac1 and Rac2 also induced the formation of thick bundles of actin filaments. In contrast, transfection with TC10 or Chp resulted in the formation of focal adhesion-like structures, whereas Wrch-1 induced long and thin filopodia. Transfection with RhoA, RhoB or RhoC induced the assembly of stress fibres, whereas Rnd1-Rnd3 resulted in the loss of stress fibres, but this effect was associated with the formation of actin- and ezrin-containing dorsal microvilli. Cells expressing RhoD and Rif had extremely long and flexible filopodia. None of the RhoBTB or Miro GTPases had any major influence on the organization of the actin filament system; instead, RhoBTB1 and RhoBTB2 were present in vesicular structures, and Miro-1 and Miro-2 were present in mitochondria. Collectively, the data obtained in this study to some extent confirm earlier observations, but also allow the identification of previously undetected roles of the different members of the Rho GTPases.

Place, publisher, year, edition, pages
2004. Vol. 377, no Pt 2, 327-337 p.
Keyword [en]
Animals, Binding Sites, COS Cells, Cells; Cultured, Comparative Study, Microfilaments/*ultrastructure, Protein Structure; Tertiary, Research Support; Non-U.S. Gov't, Swine, cdc42 GTP-Binding Protein/physiology, rac GTP-Binding Proteins/physiology, rho GTP-Binding Proteins/chemistry/classification/*physiology
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-73079PubMedID: 14521508OAI: oai:DiVA.org:uu-73079DiVA: diva2:100990
Available from: 2005-05-31 Created: 2005-05-31 Last updated: 2009-05-20Bibliographically approved

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Aspenström, PontusFransson, ÅsaSaras, Jan

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