Cellular origin of the chemokinetic inhibitor of polymorphononuclear leucocytes found in sera from patients with chronic lymphocytic leukaemia
1983 (English)In: Scandinavian journal of haematology, ISSN 0036-553X, Vol. 31, no 2, 184-192 p.Article in journal (Other academic) Published
In a previous study, we demonstrated the existence in serum from patients with chronic lymphocytic leukaemia (CLL) of a cell-directed, heat-labile chemokinetic inhibitor of polymorphonuclear leucocytes (PMN). The aim of this investigation was to study the cellular origin of this inhibitor. Supernatants from short term cultured lymphocytes from 3 healthy persons and 5 patients with CLL, and from 16 different established human non-haematopoietic and haematopoietic cell lines were analyzed. Only the leukaemic B-lymphocytes from the CLL patients produced the inhibitor. The presence of the inhibitor was also demonstrated in supernatants from the fresh CLL cells from one CLL patient and a leukaemic cell line established from these cells using Epstein-Barr virus as the immortalizing agent. A lymphoblastoid cell line derived from the normal B-cells of the same CLL patient did not produce the chemokinetic inhibitor. We therefore conclude that the cellular origin of the heat-labile, cell-directed chemokinetic inhibitor of PMN migration is the CLL cell.
Place, publisher, year, edition, pages
1983. Vol. 31, no 2, 184-192 p.
B-Lymphocytes/physiology, Carcinoma; Small Cell/physiopathology, Cell Line, Cell Transformation; Viral, Cells; Cultured, Chemotaxis; Leukocyte, Female, Herpesvirus 4; Human/genetics, Humans, Leukemia/physiopathology, Leukemia; Lymphocytic/*blood/physiopathology, Lung Neoplasms/physiopathology, Lymphoma; Large-Cell/physiopathology, Male, Neutrophils/*physiology, Research Support; Non-U.S. Gov't
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-73261PubMedID: 6310732OAI: oai:DiVA.org:uu-73261DiVA: diva2:101171