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Microdialysis-evaluated myocardial cyclooxygenase-mediated inflammation and early circulatory depression in porcine endotoxemia
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences. (Anaesthesiology and Intensive Care)
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences. (Akut- och internmedicin)
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2003 (English)In: Critical Care Medicine, ISSN 0090-3493, E-ISSN 1530-0293, Vol. 31, no 6, 1780-1785 p.Article in journal (Refereed) Published
Abstract [en]

OBJECTIVE: To evaluate the early myocardial biochemical inflammatory response with the microdialysis technique during porcine endotoxemia and to simultaneously monitor systemic hemodynamics.

DESIGN: Prospective, randomized, placebo-controlled trial with parallel groups.

SETTING: Animal research laboratory at the University Hospital of Uppsala, Sweden.

SUBJECTS: Thirteen piglets aged 12-14 wks receiving general anesthesia.

INTERVENTIONS: After thoracotomy and the insertion of microdialysis probes in standardized locations in the left ventricle of the heart and in the quadriceps muscle, seven pigs received a continuous infusion of endotoxin, initiating a severe endotoxemic shock. Six pigs received saline instead of endotoxin.

MEASUREMENTS AND MAIN RESULTS: Endotoxemia caused a rapid and pronounced elevation of a metabolite obtained from prostaglandin degradation, 15-keto-dihydro-PGF(2alpha), in myocardial microdialysate fluid being specific of cyclooxygenase (COX)-mediated inflammation (p <.001 vs. saline-infused controls). Simultaneously, we observed a decrease in left ventricular stroke work index in the endotoxemic pigs (p <.01 vs. saline-infused controls). Endotoxemia did not alter 15-keto-dihydro-PGF(2alpha) levels in quadriceps muscle. Endotoxemia caused increases in taurine, hypoxanthine, and magnesium in myocardial microdialysate (p <.05 vs. saline-infused controls), whereas the contents of pyruvate, lactate, inosine, adenosine, and calcium were not significantly changed.

CONCLUSION: Endotoxemia induced a myocardial COX-mediated inflammation without signs of ischemia. In parallel, a depletion of myocardial energy substrates and a deterioration in myocardial performance were seen.

Place, publisher, year, edition, pages
2003. Vol. 31, no 6, 1780-1785 p.
Keyword [en]
Analysis of Variance, Animals, Dinoprost/*analogs & derivatives/metabolism, Endotoxemia/immunology, Female, Hemodynamic Processes/*immunology, Inflammation/*metabolism/microbiology, Male, Microdialysis, Myocardium/immunology/*metabolism, Prostaglandin-Endoperoxide Synthase/immunology/*metabolism, Random Allocation, Research Support; Non-U.S. Gov't, Shock; Septic/*immunology, Swine
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-73273DOI: 10.1097/01.CCM.0000075740.61294.a6PubMedID: 12794420OAI: oai:DiVA.org:uu-73273DiVA: diva2:101183
Available from: 2007-03-13 Created: 2007-03-13 Last updated: 2010-10-21Bibliographically approved

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Lind, LarsLarsson, AndersNordgren, AndersBasu, Samar
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