Microdialysis-evaluated myocardial cyclooxygenase-mediated inflammation and early circulatory depression in porcine endotoxemia
2003 (English)In: Critical Care Medicine, ISSN 0090-3493, E-ISSN 1530-0293, Vol. 31, no 6, 1780-1785 p.Article in journal (Refereed) Published
OBJECTIVE: To evaluate the early myocardial biochemical inflammatory response with the microdialysis technique during porcine endotoxemia and to simultaneously monitor systemic hemodynamics.
DESIGN: Prospective, randomized, placebo-controlled trial with parallel groups.
SETTING: Animal research laboratory at the University Hospital of Uppsala, Sweden.
SUBJECTS: Thirteen piglets aged 12-14 wks receiving general anesthesia.
INTERVENTIONS: After thoracotomy and the insertion of microdialysis probes in standardized locations in the left ventricle of the heart and in the quadriceps muscle, seven pigs received a continuous infusion of endotoxin, initiating a severe endotoxemic shock. Six pigs received saline instead of endotoxin.
MEASUREMENTS AND MAIN RESULTS: Endotoxemia caused a rapid and pronounced elevation of a metabolite obtained from prostaglandin degradation, 15-keto-dihydro-PGF(2alpha), in myocardial microdialysate fluid being specific of cyclooxygenase (COX)-mediated inflammation (p <.001 vs. saline-infused controls). Simultaneously, we observed a decrease in left ventricular stroke work index in the endotoxemic pigs (p <.01 vs. saline-infused controls). Endotoxemia did not alter 15-keto-dihydro-PGF(2alpha) levels in quadriceps muscle. Endotoxemia caused increases in taurine, hypoxanthine, and magnesium in myocardial microdialysate (p <.05 vs. saline-infused controls), whereas the contents of pyruvate, lactate, inosine, adenosine, and calcium were not significantly changed.
CONCLUSION: Endotoxemia induced a myocardial COX-mediated inflammation without signs of ischemia. In parallel, a depletion of myocardial energy substrates and a deterioration in myocardial performance were seen.
Place, publisher, year, edition, pages
2003. Vol. 31, no 6, 1780-1785 p.
Analysis of Variance, Animals, Dinoprost/*analogs & derivatives/metabolism, Endotoxemia/immunology, Female, Hemodynamic Processes/*immunology, Inflammation/*metabolism/microbiology, Male, Microdialysis, Myocardium/immunology/*metabolism, Prostaglandin-Endoperoxide Synthase/immunology/*metabolism, Random Allocation, Research Support; Non-U.S. Gov't, Shock; Septic/*immunology, Swine
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-73273DOI: 10.1097/01.CCM.0000075740.61294.a6PubMedID: 12794420OAI: oai:DiVA.org:uu-73273DiVA: diva2:101183