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Orexin-A-induced Ca2+ entry: evidence for involvement of trpc channels and protein kinase C regulation.
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2005 (English)In: J Biol Chem, ISSN 0021-9258, Vol. 280, no 3, 1771-81 p.Article in journal (Refereed) Published
Abstract [en]

The orexins are peptide transmitters/hormones, which exert stimulatory actions in many types of cells via the G-protein-coupled OX(1) and OX(2) receptors. Our previous results have suggested that low (subnanomolar) concentrations of orexin-A activate Ca(2+) entry, whereas higher concentrations activate phospholipase C, Ca(2+) release, and capacitative Ca(2+) entry. As shown here, the Ca(2+) response to subnanomolar orexin-A concentrations was blocked by activation of protein kinase C by using different approaches (12-O-tetradecanoylphorbol acetate, dioctanoylglycerol, and diacylglycerol kinase inhibition) and protein phosphatase inhibition by calyculin A. The Ca(2+) response to subnanomolar orexin-A concentrations was also blocked by Mg(2+), dextromethorphan, and tetraethylammonium. These treatments neither affected the response to high concentrations of orexin-A nor the thapsigargin-stimulated capacitative entry. The capacitative entry was instead strongly suppressed by SKF96365. An inward membrane current activated by subnanomolar concentrations of orexin-A and the currents activated upon transient expression of trpc3 channels were also sensitive to Mg(2+), dextromethorphan, and tetraethylammonium. Responses to subnanomolar concentrations of orexin-A (Ca(2+) elevation, inward current, and membrane depolarization) were voltage-dependent with a loss of the response around -15 mV. By using reverse transcription-PCR, mRNA for the trpc1-4 channel isoforms were detected in the CHO-hOX1-C1 cells. The expression of truncated TRPC channel isoforms, in particular trpc1 and trpc3, reduced the response to subnanomolar concentrations of orexin-A but did not affect the response to higher concentrations of orexin-A. The results suggest that activation of the OX(1) receptor leads to opening of a Ca(2+)-permeable channel, involving trpc1 and -3, which is controlled by protein kinase C.

Place, publisher, year, edition, pages
2005. Vol. 280, no 3, 1771-81 p.
Keyword [en]
Animals, Base Sequence, CHO Cells, Calcium/*metabolism, DNA Primers, Hamsters, Immunoprecipitation, Intracellular Signaling Peptides and Proteins/*pharmacology, Ion Channels/*physiology, Ion Transport, Magnesium/metabolism, Molecular Sequence Data, Neuropeptides/*pharmacology, Patch-Clamp Techniques, Protein Kinase C/*metabolism, Research Support; Non-U.S. Gov't
URN: urn:nbn:se:uu:diva-73297PubMedID: 15537648OAI: oai:DiVA.org:uu-73297DiVA: diva2:101207
Available from: 2005-06-01 Created: 2005-06-01 Last updated: 2011-01-11

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Kukkonen, JyrkiAkerman, Karl
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Department of Neuroscience

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