uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Explicit formulation of different receptor-G-protein interactions and effector regulation.
Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Neuroscience. Fysiologi. (Kukkonen)
2004 (English)In: Bioinformatics, ISSN 1367-4803, Vol. 20, no 15, 2411-20 p.Article in journal (Refereed) Published
Abstract [en]

MOTIVATION: G-protein-coupled receptors (GPCRs) can create different intracellular signals depending on which G-proteins they couple to and which intracellular signal-integrators, such as adenylyl cyclases, are expressed in the cell. One and the same GPCR can activate multiple G-protein species, generating signals, which either inhibit or amplify each other. Because of this complexity, extraction of mechanistic information from concentration-response curves is not straightforward. RESULTS: To tackle this problem, I describe in this paper explicit equations for GPCR-interaction with two G-protein species by different possible mechanisms, and also an equation for the regulation of an effector enzyme by the activated G-proteins or their effectors. Arithmetic solutions to these equations are presented, which resulted in the equations being applicable in a spreadsheet program environment. These equations are useful in simulations to analyze results, to design experiments and to test hypotheses. Some examples of this are presented in this study.

Place, publisher, year, edition, pages
2004. Vol. 20, no 15, 2411-20 p.
Keyword [en]
Adaptation; Physiological/physiology, Binding Sites, Comparative Study, Computer Simulation, GTP-Binding Proteins/*chemistry/*metabolism, Models; Biological, Models; Chemical, Protein Binding, Protein Interaction Mapping/*methods, Receptors; G-Protein-Coupled/*chemistry/*metabolism, Research Support; Non-U.S. Gov't, Signal Transduction/physiology
URN: urn:nbn:se:uu:diva-73300PubMedID: 15087317OAI: oai:DiVA.org:uu-73300DiVA: diva2:101210
Available from: 2005-06-01 Created: 2005-06-01 Last updated: 2011-01-12

Open Access in DiVA

No full text

Other links


Search in DiVA

By author/editor
Kukkonen, Jyrki
By organisation
Department of Neuroscience

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 169 hits
ReferencesLink to record
Permanent link

Direct link