Explicit formulation of different receptor-G-protein interactions and effector regulation.
2004 (English)In: Bioinformatics, ISSN 1367-4803, Vol. 20, no 15, 2411-20 p.Article in journal (Refereed) Published
MOTIVATION: G-protein-coupled receptors (GPCRs) can create different intracellular signals depending on which G-proteins they couple to and which intracellular signal-integrators, such as adenylyl cyclases, are expressed in the cell. One and the same GPCR can activate multiple G-protein species, generating signals, which either inhibit or amplify each other. Because of this complexity, extraction of mechanistic information from concentration-response curves is not straightforward. RESULTS: To tackle this problem, I describe in this paper explicit equations for GPCR-interaction with two G-protein species by different possible mechanisms, and also an equation for the regulation of an effector enzyme by the activated G-proteins or their effectors. Arithmetic solutions to these equations are presented, which resulted in the equations being applicable in a spreadsheet program environment. These equations are useful in simulations to analyze results, to design experiments and to test hypotheses. Some examples of this are presented in this study.
Place, publisher, year, edition, pages
2004. Vol. 20, no 15, 2411-20 p.
Adaptation; Physiological/physiology, Binding Sites, Comparative Study, Computer Simulation, GTP-Binding Proteins/*chemistry/*metabolism, Models; Biological, Models; Chemical, Protein Binding, Protein Interaction Mapping/*methods, Receptors; G-Protein-Coupled/*chemistry/*metabolism, Research Support; Non-U.S. Gov't, Signal Transduction/physiology
IdentifiersURN: urn:nbn:se:uu:diva-73300PubMedID: 15087317OAI: oai:DiVA.org:uu-73300DiVA: diva2:101210