uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Renal hyaluronan accumulation and hyaluronan synthase expression after ischaemia-reperfusion injury in the rat.
Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Cell Biology.
Department of Surgical Sciences.
Department of Medical Sciences.
Ludwiginstitutet för Cancerforskning.
Show others and affiliations
2004 (English)In: Nephrol Dial Transplant, ISSN 0931-0509, Vol. 19, no 4, 823-30 p.Article in journal (Refereed) Published
Abstract [en]

BACKGROUND: Hyaluronan (HA) is a connective tissue component with unique water binding and pro-inflammatory properties. It has been suggested that HA is involved in normal renal water handling but also in several pathological conditions such as organ rejection and ischaemia-reperfusion (IR) injury. METHODS: In anaesthetized normal rats we investigated if renal cortical HA accumulation and the intrarenal distribution and expression of HA synthases (Has 1, 2 and 3) correlate with renal dysfunction after renal IR injury. After 20, 30 or 45 min of unilateral renal ischaemia and 72 h of reperfusion, renal function and cortical HA content were measured. Has 1, 2 and 3 mRNA were determined in control and IR kidneys subjected to 45 min ischaemia and 72 h reperfusion. RESULTS: IR kidneys had reduced urine concentrating ability, potassium excretion, glomerular filtration rate (GFR) and renal blood flow. On average, IR kidneys had more than 10 times higher amounts of cortical HA than the contralateral control kidney and their water content was elevated while medullary HA was largely unaffected. Has 2 expression in the cortex was heavily up-regulated in IR kidneys while Has 3 remained at control levels. Has 1 could never be detected. There was a direct correlation between the amount of cortical HA and the time period of ischaemia and also between the cortical amount of HA and depression of functional parameters. CONCLUSIONS: IR injury depresses parameters of renal function, which coincides with an elevated cortical HA content and Has 2 expression. The enhanced Has 2 expression indicates that the cortical HA accumulation is primarily dependent on increased HA synthesis and not impaired degradation/elimination. The water binding and pro-inflammatory properties of HA may contribute to renal dysfunction after IR.

Place, publisher, year, edition, pages
2004. Vol. 19, no 4, 823-30 p.
Keyword [en]
Animals, Hyaluronic Acid/*metabolism, Kidney/*metabolism/pathology, Male, Rats, Rats; Sprague-Dawley, Reperfusion Injury/*metabolism, Research Support; Non-U.S. Gov't, Transferases/*biosynthesis
URN: urn:nbn:se:uu:diva-73375PubMedID: 15031336OAI: oai:DiVA.org:uu-73375DiVA: diva2:101285
Available from: 2007-03-26 Created: 2007-03-26 Last updated: 2011-01-12

Open Access in DiVA

No full text

Other links


Search in DiVA

By author/editor
Johnsson, CeciliaJacobson, AnnicaHällgren, RogerHansell, Peter
By organisation
Department of Medical Cell BiologyDepartment of Surgical SciencesDepartment of Medical Sciences

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 265 hits
ReferencesLink to record
Permanent link

Direct link