Simultaneous detection of both nitric oxide and reactive oxygen species in guinea pig vestibular sensory cells
2002 (English)In: Journal for Oto-Rhino-Laryngology, ISSN 0301-1569, Vol. 64, no 2, 143-147 p.Article in journal (Other (popular science, discussion, etc.)) Published
Gentamicin-induced production of reactive oxygen species (ROS) and of nitric oxide (NO) in the vestibular end organs of the guinea pig was investigated by applying two new fluorescence indicators, 4,5-diaminofluorescein diacetate for direct detection of NO and dihydrotetramethylrosamine for ROS. The vestibular sensory cells produced both NO and ROS after exposure to gentamicin. A nonspecific inhibitor of NO synthase, L-NAME, inhibited the production of NO but did not appear to affect the production of ROS following exposure to gentamicin. In contrast, a radical scavenger, D-methionine, or the neurotrophin BDNF suppressed the production of ROS, in turn stimulating NO production. These findings could indicate that both NO and ROS play an important role in aminoglycoside ototoxicity.
Place, publisher, year, edition, pages
2002. Vol. 64, no 2, 143-147 p.
Nitric oxide, Reactive oxygen species, Fluorescence, Vestibular organ, Gentamicin, Ototoxicity
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-73577DOI: 10.1159/000057794PubMedID: 12021507OAI: oai:DiVA.org:uu-73577DiVA: diva2:101487