uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Inhibition of Bacterial RNase P RNA by Phenothiazine Derivatives
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology.
2016 (English)In: Biomolecules, ISSN 0045-2068, E-ISSN 2218-273X, Vol. 6, no 3, 38Article in journal (Refereed) Published
Abstract [en]

There is a need to identify novel scaffolds and targets to develop new antibiotics. Methylene blue is a phenothiazine derivative, and it has been shown to possess anti-malarial and anti-trypanosomal activities. Here, we show that different phenothiazine derivatives and pyronine G inhibited the activities of three structurally different bacterial RNase P RNAs (RPRs), including that from Mycobacterium tuberculosis, with K-i values in the lower mu M range. Interestingly, three antipsychotic phenothiazines (chlorpromazine, thioridazine, and trifluoperazine), which are known to have antibacterial activities, also inhibited the activity of bacterial RPRs, albeit with higher Ki values than methylene blue. Phenothiazines also affected lead(II)-induced cleavage of bacterial RPR and inhibited yeast tRNAPhe, indicating binding of these drugs to functionally important regions. Collectively, our findings provide the first experimental data showing that long, noncoding RNAs could be targeted by different phenothiazine derivatives.

Place, publisher, year, edition, pages
2016. Vol. 6, no 3, 38
Keyword [en]
RNA processing, catalytic RNA, RNase P, phenothiazines
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-304162DOI: 10.3390/biom6030038ISI: 000382578800010OAI: oai:DiVA.org:uu-304162DiVA: diva2:1014905
Available from: 2016-10-03 Created: 2016-10-03 Last updated: 2017-11-30Bibliographically approved

Open Access in DiVA

fulltext(2687 kB)45 downloads
File information
File name FULLTEXT01.pdfFile size 2687 kBChecksum SHA-512
1c8bcbfe42e861f34a125b5981ed2f85b8790f239cbab49b2c55237a4a677fdf9cf410daf6111b16f8462ce18aa9e7e769522be3e137a925b72b5772f243f6d5
Type fulltextMimetype application/pdf

Other links

Publisher's full text

Search in DiVA

By author/editor
Mao, GuanzhongKirsebom, Leif A.
By organisation
Department of Cell and Molecular Biology
In the same journal
Biomolecules
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
Total: 45 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 405 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf