The infectious particle of insect-borne totivirus-like Omono River virus has raised ridges and lacks fibre complexes
2016 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 6, 33170Article in journal (Refereed) Published
Omono River virus (OmRV) is a double-stranded RNA virus isolated from Culex mosquitos, and it belongs to a group of unassigned insect viruses that appear to be related to Totiviridae. This paper describes electron cryo-microscopy (cryoEM) structures for the intact OmRV virion to 8.9 angstrom resolution and the structure of the empty virus-like-particle, that lacks RNA, to 8.3 angstrom resolution. The icosahedral capsid contains 120-subunits and resembles another closely related arthropod-borne totivirus-like virus, the infectious myonecrosis virus (IMNV) from shrimps. Both viruses have an elevated plateau around their icosahedral 5-fold axes, surrounded by a deep canyon. Sequence and structural analysis suggests that this plateau region is mainly composed of the extended C-terminal region of the capsid proteins. In contrast to IMNV, the infectious form of OmRV lacks extensive fibre complexes at its 5-fold axes as directly confirmed by a contrast-enhancement technique, using Zernike phase-contrast cryo-EM. Instead, these fibre complexes are replaced by a short "plug" structure at the five-fold axes of OmRV. OmRV and IMNV have acquired an extracellular phase, and the structures at the five-fold axes may be significant in adaptation to cell-to-cell transmission in metazoan hosts.
Place, publisher, year, edition, pages
2016. Vol. 6, 33170
IdentifiersURN: urn:nbn:se:uu:diva-304148DOI: 10.1038/srep33170ISI: 000382864800001PubMedID: 27616740OAI: oai:DiVA.org:uu-304148DiVA: diva2:1014955
FunderSwedish Research Council, 62820081109 822-2010-6157 822-2012-5260 828-2012-108Knut and Alice Wallenberg Foundation, KAW-2011.081EU, European Research Council, ERC-291602The Swedish Foundation for International Cooperation in Research and Higher Education (STINT), JA2014-5721Science for Life Laboratory - a national resource center for high-throughput molecular bioscience