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Choice of molecular barcode will affect species prevalence but not bacterial community composition
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Ecology and Genetics, Limnology.
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2016 (English)In: Marine Genomics, ISSN 1874-7787, E-ISSN 1876-7478, Vol. 29, 39-43 p.Article in journal (Refereed) Published
Abstract [en]

Abstract The rapid advancement of next generation sequencing protocols in recent years has led to the diversification in the methods used to study microbial communities; however, how comparable the data generated from these different methods are, remains unclear. In this study we compared the taxonomic composition and seasonal dynamics of the bacterial community determined by two distinct 16s amplicon sequencing protocols: sequencing of the V6 region of the 16s rRNA gene using 454 pyrosequencing vs the V4 region of the 16s rRNA gene using the Illumina Hiseq 2500 platform. Significant differences between relative abundances at all taxonomic levels were observed; however, their seasonal dynamics between phyla were largely consistent between methods. This study highlights that care must be taken when comparing datasets generated from different methods.

Place, publisher, year, edition, pages
2016. Vol. 29, 39-43 p.
Keyword [en]
Marine bacterial community, 16S, Pyrosequencing, Illumina
National Category
Biological Sciences
URN: urn:nbn:se:uu:diva-304305DOI: 10.1016/j.margen.2016.09.001OAI: oai:DiVA.org:uu-304305DiVA: diva2:1015059
Available from: 2016-10-04 Created: 2016-10-04 Last updated: 2016-10-04

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Publisher's full texthttp://www.sciencedirect.com/science/article/pii/S1874778716300915

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