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Optimized indirect (76)Br-bromination of antibodies using N-succinimidyl para-[76Br]bromobenzoate for radioimmuno PET
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Oncology, Radiology and Clinical Immunology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Oncology, Radiology and Clinical Immunology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Oncology, Radiology and Clinical Immunology.ORCID iD: 0000-0001-6120-2683
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Oncology, Radiology and Clinical Immunology.
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2000 (English)In: Nuclear Medicine and Biology, ISSN 0969-8051, E-ISSN 1872-9614, Vol. 27, no 8, 837-43 p.Article in journal (Refereed) Published
Abstract [en]

Monoclonal antibody 38S1 was radiobrominated with the positron emitter (76)Br (T(1/2) = 16.2 h). Indirect labeling was performed using N-succinimidyl para-(tri-methylstannyl)benzoate (SPMB) as the precursor molecule. SPMB was labeled using Chloramine-T yielding N-succinimidyl para-[(76)Br]bromobenzoate, which was then conjugated to the antibody. Optimization of the labeling conditions and further conjugation gave a total yield ( mean+/-max error) of 49+/-2%. The immunoreactivity of the antibodies was retained after labeling. Thus, antibodies intended for positron emission tomography can be labeled with (76)Br, which gives high yields and preserved immunoreactivity when using the SPMB technique described.

Place, publisher, year, edition, pages
2000. Vol. 27, no 8, 837-43 p.
Keyword [en]
Antibodies; Monoclonal/*chemistry, Benzoates/*chemistry, Bromine Radioisotopes, Carcinoembryonic Antigen/chemistry/immunology, Indicators and Reagents, Isotope Labeling, Radiopharmaceuticals/*chemical synthesis, Research Support; Non-U.S. Gov't, Succinimides/*chemistry, Tomography; Emission-Computed
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Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-73664DOI: 10.1016/S0969-8051(00)00153-0PubMedID: 11150718OAI: oai:DiVA.org:uu-73664DiVA: diva2:101574
Available from: 2005-06-13 Created: 2005-06-13 Last updated: 2017-12-14Bibliographically approved

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Tolmachev, VladimirOrlova, AnnaLundqvist, HansSundin, Anders

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