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Multi-parameter investigation of tandem mass spectrometry in a linear ion trap using response surface modelling
Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Chemistry. Analytisk kemi.
Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Chemistry. Analytisk kemi.
2005 (English)In: Journal of Mass Spectrometry, no 40, 317-324 p.Article in journal (Refereed) Published
Abstract [en]

The feasibility of experimental design in combination with subsequent response surface modelling was illustrated for the prediction and interpretation of tandem mass spectrometric (MS/MS) fragmentation data using a linear quadrupole ion trap under various experimental conditions. The instrumental parameters included were (i) the pressure of the collision gas, (ii) the collision energy, (iii) the fill time of the linear ion trap and (iv) the scan rate. The spectral intensity and width of five fragment ions of the doubly charged neuro-active peptide bombesin were used for evaluation, and all experiments were performed so as to resemble the results obtained from a liquid chromatographic peak. The reported results show how fairly simple mathematical tools can be utilized sucessfully to describe fundamental mechanisms associated with multiple collisional activation and collision-induced dissociation processes without an extensively controlled experimental environment. Most beneficial, using the suggested approach, is the ability to study interaction (synergistic) effects between various parameters. As we realized from the results, many interaction effects are indeed significant. For example, the effect on the signal intensity of different collision gas pressure settings is strongly dependent on the settings of the other parameters. The described approach can easily be adopted for optimization purposes of any MS/MS experiment.

Place, publisher, year, edition, pages
2005. no 40, 317-324 p.
Keyword [en]
collision-induced dissociation, tandem mass spectrometry, linear ion trap, optimization, response surface modelling
Identifiers
URN: urn:nbn:se:uu:diva-73849OAI: oai:DiVA.org:uu-73849DiVA: diva2:101760
Available from: 2005-07-07 Created: 2005-07-07 Last updated: 2011-01-11

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