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Ribosome formation from subunits studied by stopped-flow and Rayleigh light scattering
Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Cell and Molecular Biology. Molekylärbiologi.
Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Cell and Molecular Biology. Molekylärbiologi.
Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Cell and Molecular Biology. Molekylärbiologi.
2004 (English)In: Biol Procedure Online, Vol. 6, 35–54- p.Article in journal (Refereed) Published
Abstract [en]

Light scattering and standard stopped-flow techniques were used to monitor rapid association of ribosomal subunits during initiation of eubacterial protein synthesis. The effects of the initiation factors IF1, IF2, IF3 and buffer conditions on subunit association were studied along with the role of GTP in this process. The part of light scattering theory that is essential for kinetic measurements is high-lighted in the main text and a more general treatment of Rayleigh scattering from macromolecules is given in an appendix.

Place, publisher, year, edition, pages
2004. Vol. 6, 35–54- p.
Keyword [en]
Ribosomes, Prokaryotic Initiation Factor-2, Scattering, Radiation
Identifiers
URN: urn:nbn:se:uu:diva-74171OAI: oai:DiVA.org:uu-74171DiVA: diva2:102081
Available from: 2005-08-31 Created: 2005-08-31 Last updated: 2011-01-12

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Antoun, AymanPavlov, MichaelEhrenberg, Måns

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