A Time-resolved Investigation of Ribosomal Subunit Association
2005 (English)In: Journal of Molecular Biology, Vol. 346, no 5, 1243-1258 p.Article in journal (Refereed) Published
The notion that the ribosome is dynamic has been supported by various biochemical techniques, as well as by differences observed in high-resolution structures of ribosomal complexes frozen in various functional states. Yet, the mechanisms and extent of rRNA dynamics are still largely unknown. We have used a novel, fast chemical-modification technique to provide time-resolved details of 16 S rRNA structural changes that occur as bridges are formed between the ribosomal subunits as they associate. Association of different 16 S rRNA regions was found to be a sequential, multi-step process involving conformational rearrangements within the 30 S subunit. Our results suggest that key regions of 16 S rRNA, necessary for decoding and tRNA A-site binding, are structurally altered in a time-dependent manner by association with the 50 S ribosomal subunits.
Place, publisher, year, edition, pages
2005. Vol. 346, no 5, 1243-1258 p.
ribosome, dynamics, structure, RNA, subunit association
IdentifiersURN: urn:nbn:se:uu:diva-74172DOI: doi:10.1016/j.jmb.2004.12.054OAI: oai:DiVA.org:uu-74172DiVA: diva2:102082