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A Time-resolved Investigation of Ribosomal Subunit Association
Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Cell and Molecular Biology. Molekylärbiologi.
Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Cell and Molecular Biology. Molekylärbiologi.
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2005 (English)In: Journal of Molecular Biology, Vol. 346, no 5, 1243-1258 p.Article in journal (Refereed) Published
Abstract [en]

The notion that the ribosome is dynamic has been supported by various biochemical techniques, as well as by differences observed in high-resolution structures of ribosomal complexes frozen in various functional states. Yet, the mechanisms and extent of rRNA dynamics are still largely unknown. We have used a novel, fast chemical-modification technique to provide time-resolved details of 16 S rRNA structural changes that occur as bridges are formed between the ribosomal subunits as they associate. Association of different 16 S rRNA regions was found to be a sequential, multi-step process involving conformational rearrangements within the 30 S subunit. Our results suggest that key regions of 16 S rRNA, necessary for decoding and tRNA A-site binding, are structurally altered in a time-dependent manner by association with the 50 S ribosomal subunits.

Place, publisher, year, edition, pages
2005. Vol. 346, no 5, 1243-1258 p.
Keyword [en]
ribosome, dynamics, structure, RNA, subunit association
Identifiers
URN: urn:nbn:se:uu:diva-74172DOI: doi:10.1016/j.jmb.2004.12.054OAI: oai:DiVA.org:uu-74172DiVA: diva2:102082
Available from: 2005-08-31 Created: 2005-08-31 Last updated: 2011-01-11

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Publisher's full texthttp://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WK7-4F8BDS9-1&_coverDate=03%2F11%2F2005&_alid=308923022&_rdoc=1&_fmt=&_orig=search&_qd=1&_cdi=6899&_sort=d&view=c&_acct=C000035158&_version=1&_urlVersion=0&_userid=651519&md5=2dfe2d2321aed20e33210fd52731ef86

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Antoun, AymanEhrenberg, Måns

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