The influence of IL-3, IL-5, and GM-CSF on normal human eosinophil and neutrophil C3b-induced degranulation
1993 (English)In: Allergy. European Journal of Allergy and Clinical Immunology, ISSN 0105-4538, E-ISSN 1398-9995, Vol. 48, no 6, 437-442 p.Article in journal (Refereed) Published
The priming effect of interleukin-3 (IL-3), interleukin-5 (IL-5), and granulocyte/macrophage-colony stimulating factor (GM-CSF) on eosinophil and neutrophil degranulation was studied. Granulocytes were obtained from normal donors, and degranulation was induced by incubation with serum-opsonized Sephadex particles. The released amounts of eosinophil cationic protein (ECP), eosinophil protein X (EPX), myeloperoxidase (MPO), and lactoferrin (LF) were measured by radioimmunoassay (RIA). The effect of IL-5 was dose- and time-dependent, with a maximal enhancement of ECP and EPX release of 71% (P < 0.03) and 66% (P < 0.03), respectively. Neutrophil degranulation, however, was unaffected. IL-3 was marginally effective, whereas GM-CSF seemed to act as a secretagogue for both eosinophil and neutrophil degranulation. We conclude that IL-5 selectively primes eosinophil degranulation, whereas IL-3 and GM-CSF seem to act as secretagogues for eosinophils and neutrophils. The results indicate that IL-5 may be involved in the priming of eosinophils as observed in patients with asthma and hypereosinophilic syndrome (HES).
Place, publisher, year, edition, pages
1993. Vol. 48, no 6, 437-442 p.
Blood Proteins/drug effects/secretion, Cell Degranulation/*drug effects, Comparative Study, Complement 3b/pharmacology, Eosinophil Granule Proteins, Eosinophil-Derived Neurotoxin, Eosinophils/drug effects/*physiology, Granulocyte-Macrophage Colony-Stimulating Factor/*pharmacology, Humans, Interleukin-3/*pharmacology, Interleukin-5/*pharmacology, Neutrophils/drug effects/*physiology, Research Support; Non-U.S. Gov't, Ribonucleases
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-74322PubMedID: 8238799OAI: oai:DiVA.org:uu-74322DiVA: diva2:102232