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The influence of IL-3, IL-5, and GM-CSF on normal human eosinophil and neutrophil C3b-induced degranulation
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences.
1993 (English)In: Allergy. European Journal of Allergy and Clinical Immunology, ISSN 0105-4538, E-ISSN 1398-9995, Vol. 48, no 6, 437-442 p.Article in journal (Refereed) Published
Abstract [en]

The priming effect of interleukin-3 (IL-3), interleukin-5 (IL-5), and granulocyte/macrophage-colony stimulating factor (GM-CSF) on eosinophil and neutrophil degranulation was studied. Granulocytes were obtained from normal donors, and degranulation was induced by incubation with serum-opsonized Sephadex particles. The released amounts of eosinophil cationic protein (ECP), eosinophil protein X (EPX), myeloperoxidase (MPO), and lactoferrin (LF) were measured by radioimmunoassay (RIA). The effect of IL-5 was dose- and time-dependent, with a maximal enhancement of ECP and EPX release of 71% (P < 0.03) and 66% (P < 0.03), respectively. Neutrophil degranulation, however, was unaffected. IL-3 was marginally effective, whereas GM-CSF seemed to act as a secretagogue for both eosinophil and neutrophil degranulation. We conclude that IL-5 selectively primes eosinophil degranulation, whereas IL-3 and GM-CSF seem to act as secretagogues for eosinophils and neutrophils. The results indicate that IL-5 may be involved in the priming of eosinophils as observed in patients with asthma and hypereosinophilic syndrome (HES).

Place, publisher, year, edition, pages
1993. Vol. 48, no 6, 437-442 p.
Keyword [en]
Blood Proteins/drug effects/secretion, Cell Degranulation/*drug effects, Comparative Study, Complement 3b/pharmacology, Eosinophil Granule Proteins, Eosinophil-Derived Neurotoxin, Eosinophils/drug effects/*physiology, Granulocyte-Macrophage Colony-Stimulating Factor/*pharmacology, Humans, Interleukin-3/*pharmacology, Interleukin-5/*pharmacology, Neutrophils/drug effects/*physiology, Research Support; Non-U.S. Gov't, Ribonucleases
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Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-74322PubMedID: 8238799OAI: oai:DiVA.org:uu-74322DiVA: diva2:102232
Available from: 2005-09-21 Created: 2005-09-21 Last updated: 2017-12-14Bibliographically approved

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Carlson, MPeterson, CVenge, Per

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