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Human eosinophil peroxidase: purification and characterization
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences.
1985 (English)In: Journal of Immunology, ISSN 0022-1767, E-ISSN 1550-6606, Vol. 134, no 3, 1875-1879 p.Article in journal (Refereed) Published
Abstract [en]

Human eosinophil peroxidase (EPO) was isolated from granules from granulocytes of a patient with hypereosinophilia. The granules were extracted by means of 0.2 M NaAc, pH 4.0. The purification steps included gel filtration chromatography on Sephadex G-75 superfine and ion-exchange chromatography on CM-Sephadex G-50. The purified protein showed one band on agarose-electrophoresis, a high peroxidase activity, and a 415-nm/280 nm ratio of 1.15. After reduction, EPO showed two bands on SDS-PAGE of m.w. 52,000 and 15,000, respectively. On gel filtration, the unreduced protein had a m.w. of approximately 77,000. Amino acid analyses showed a high content of arginine and aspartic acid. Monospecific antibodies to EPO were prepared in rabbits, and a specific radioimmunoassay was developed. There was an almost linear correlation between the content of EPO measured by the radioimmunoassay and the number of eosinophils in a mixed cell extract from reference material, indicating the eosinophil origin of EPO. The content of EPO was estimated to be 15.0 micrograms/10(6) eosinophils.

Place, publisher, year, edition, pages
1985. Vol. 134, no 3, 1875-1879 p.
Keyword [en]
Amino Acids/analysis, Antigen-Antibody Reactions, Chromatography; Gel, Eosinophil Peroxidase, Eosinophilia/enzymology, Eosinophils/*enzymology, Humans, Immunodiffusion, Leukocyte Count, Molecular Weight, Peroxidases/analysis/*isolation & purification/metabolism, Radioimmunoassay, Research Support; Non-U.S. Gov't
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Medical and Health Sciences
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URN: urn:nbn:se:uu:diva-74328PubMedID: 3918110OAI: oai:DiVA.org:uu-74328DiVA: diva2:102238
Available from: 2005-09-21 Created: 2005-09-21 Last updated: 2010-06-04Bibliographically approved

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Carlson, MVenge, P

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