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Accurate monoisotopic mass measurements of peptides: Possibilities and limitations of high resolution time-of-flight particle desorption mass spectrometry
Uppsala University, Disciplinary Domain of Science and Technology, Physics, Department of Radiation Sciences.
Uppsala University, Disciplinary Domain of Science and Technology, Physics, Department of Radiation Sciences.
Uppsala University, Disciplinary Domain of Science and Technology, Physics, Department of Radiation Sciences.
1996 (English)In: Rapid Communications in Mass Spectrometry, ISSN 0951-4198, E-ISSN 1097-0231, Vol. 10, no 11, 1386-1392 p.Article in journal (Refereed) Published
Abstract [en]

The possibilities and limitations of time-of-flight particle-desorption mass spectrometry are analysed from the standpoint of accuracy of monoisotopic mass determination. For an instrument with mass reflector, typical mass accuracy was found to be ca. 20 ppm (standard deviation). The main limitation was found to be the mechanism of secondary ion production itself. A fraction of biological ions are formed in the gas phase and therefore exhibit both time delay and energy deficit. This leads to a limited resolving power and non-symmetric ion peaks. A peak shape model, taking into account gas-phase ion formation, is built and analysed, and a simple correction procedure proposed. The application of the correction has increased the mass accuracy to 12 ppm (standard deviation). It is shown that further progress cannot be achieved by a correction procedure without instrumental improvements.

Place, publisher, year, edition, pages
1996. Vol. 10, no 11, 1386-1392 p.
Keyword [en]
extraction, molecules
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:uu:diva-74474DOI: 10.1002/(SICI)1097-0231(199608)10:11<1386::AID-RCM652>3.0.CO;2-TOAI: oai:DiVA.org:uu-74474DiVA: diva2:102384
Available from: 2008-10-17 Created: 2008-10-17 Last updated: 2013-10-02Bibliographically approved

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